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A novel immuno-PCR method using cDNA display.
Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N. Anzai H, et al. Anal Biochem. 2019 Aug 1;578:1-6. doi: 10.1016/j.ab.2019.04.017. Epub 2019 Apr 25. Anal Biochem. 2019. PMID: 31028717
Immuno-PCR (IPCR) provides sensitive and versatile detection of a variety of antigens by conjugating a PCR-amplifiable DNA reporter to a specific antibody or an aptamer. ...The newly developed system here, termed cDNA display mediated immuno …
Immuno-PCR (IPCR) provides sensitive and versatile detection of a variety of antigens by conjugating a PCR-amplifiable DNA …
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method.
Jayathilake C, Terai T, Nemoto N. Jayathilake C, et al. Bio Protoc. 2019 Dec 20;9(24):e3457. doi: 10.21769/BioProtoc.3457. eCollection 2019 Dec 20. Bio Protoc. 2019. PMID: 33654952 Free PMC article.
Immuno-PCR (IPCR) is a powerful method in antigen detection where a PCR-amplifiable DNA reporter is conjugated to a specific antibody or an aptamer for the target molecule. ...The cDNA display molecule for IPCR is generated first by trans …
Immuno-PCR (IPCR) is a powerful method in antigen detection where a PCR-amplifiable DNA reporter is conjugated t …
In vitro selection of anti-gliadin single-domain antibodies from a naive library for cDNA-display mediated immuno-PCR.
Jayathilake C, Kumachi S, Arai H, Motohashi M, Terai T, Murakami A, Nemoto N. Jayathilake C, et al. Anal Biochem. 2020 Jan 15;589:113490. doi: 10.1016/j.ab.2019.113490. Epub 2019 Oct 31. Anal Biochem. 2020. PMID: 31678363
The cDNA display method is a promising in vitro display technique, which uniquely converts an unstable mRNA-protein fusion molecule to a stable mRNA/cDNA-protein fusion molecule using a well-designed puromycin linker. Three candidate VHHs were s …
The cDNA display method is a promising in vitro display technique, which uniquely converts an unstable mRNA-prot …