Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010

Arch Virol. 2015 May;160(5):1171-9. doi: 10.1007/s00705-015-2374-6. Epub 2015 Feb 25.

Abstract

Equine group A rotavirus (RVA) G3P[12] and G14P[12] strains cause gastroenteritis in foals worldwide. Both of these strains have been co-circulating in Japan since G14P[12] strains emerged in the late 1990s. Although it is important to comprehensively understand the evolution of RVA strains, whole-genome sequence data on recent equine RVA strains in Japan are lacking. Therefore, in this study, whole-genome analysis of 23 equine RVA isolates from the late 1990s and 2009-2010 and the vaccine strain RVA/Horse-tc/JPN/HO-5/1982/G3P[12] (HO-5) was performed. The G3 strains, including strain HO-5, shared a G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 genotype constellation, and all of their 11 gene segments were highly conserved, regardless of the year of isolation. G14 strains also exhibited an identical genotype constellation (G14-P[12]-I2-R2-C2-M3-A10-N2-T3-E2-H7), but, phylogenetically, segregated into two lineages within the VP7-G14 and NSP4-E2 genotypes. G14 strains were closely related to G3 strains in their VP4, VP1-3, NSP1-3 and NSP5 gene segments. Interestingly, the NSP4 gene of all G3 and G14 strains isolated in the late 1990s branched into a bovine-RVA-like NSP4 gene cluster. These results indicate that, apart from VP7, VP6, and NSP4 genes, the Japanese equine RVA strains share a highly conserved genetic backbone, and that strains possessing a bovine-RVA-like NSP4 gene were predominant in the late 1990s in Japan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cluster Analysis
  • Gastroenteritis / veterinary
  • Gastroenteritis / virology
  • Genome, Viral*
  • Horse Diseases / virology*
  • Horses
  • Japan
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics*
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / veterinary*
  • Rotavirus Infections / virology
  • Sequence Analysis, DNA*
  • Sequence Homology

Substances

  • RNA, Viral

Associated data

  • GENBANK/AB908901
  • GENBANK/AB908902
  • GENBANK/AB908903
  • GENBANK/AB908904
  • GENBANK/AB908905
  • GENBANK/AB908906
  • GENBANK/AB908907
  • GENBANK/AB908908
  • GENBANK/AB908909
  • GENBANK/AB908910
  • GENBANK/AB908911
  • GENBANK/AB908912
  • GENBANK/AB908913
  • GENBANK/AB908914
  • GENBANK/AB908915
  • GENBANK/AB908916
  • GENBANK/AB908917
  • GENBANK/AB908918
  • GENBANK/AB908919
  • GENBANK/AB908920
  • GENBANK/AB908921
  • GENBANK/AB908922
  • GENBANK/AB908923
  • GENBANK/AB908924
  • GENBANK/AB908925
  • GENBANK/AB908926
  • GENBANK/AB908927
  • GENBANK/AB908928
  • GENBANK/AB908929
  • GENBANK/AB908930
  • GENBANK/AB908931
  • GENBANK/AB908932
  • GENBANK/AB908933
  • GENBANK/AB908934
  • GENBANK/AB908935
  • GENBANK/AB908936
  • GENBANK/AB908937
  • GENBANK/AB908938
  • GENBANK/AB908939
  • GENBANK/AB908940
  • GENBANK/AB908941
  • GENBANK/AB908942
  • GENBANK/AB908943
  • GENBANK/AB908944
  • GENBANK/AB908945
  • GENBANK/AB908946
  • GENBANK/AB908947
  • GENBANK/AB908948
  • GENBANK/AB908949
  • GENBANK/AB908950
  • GENBANK/AB908951
  • GENBANK/AB908952
  • GENBANK/AB908953
  • GENBANK/AB908954
  • GENBANK/AB908955