3D scaffold-free microlivers with drug metabolic function generated by lineage-reprogrammed hepatocytes from human fibroblasts.
Lu Z, Priya Rajan SA, Song Q, Zhao Y, Wan M, Aleman J, Skardal A, Bishop C, Atala A, Lu B.
Lu Z, et al.
Biomaterials. 2021 Feb;269:120668. doi: 10.1016/j.biomaterials.2021.120668. Epub 2021 Jan 8.
Biomaterials. 2021.
PMID: 33461059
Here, we simplify preparation of reprogramming reagents by expressing six transcriptional factors (HNF4A, FOXA2, FOXA3, ATF5, PROX1, and HNF1) from two lentiviral vectors, each expressing three factors. ...To mimic the physiologic liver microenvironment and improve …
Here, we simplify preparation of reprogramming reagents by expressing six transcriptional factors (HNF4A, FOXA2, FOXA3, ATF5, PROX1, …