Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies.
Stivers JT, Abeygunawardana C, Mildvan AS, Hajipour G, Whitman CP, Chen LH.
Stivers JT, et al. Among authors: abeygunawardana c.
Biochemistry. 1996 Jan 23;35(3):803-13. doi: 10.1021/bi951077g.
Biochemistry. 1996.
PMID: 8547260
In support of this proposed role, we have found that the active-site-directed irreversible inhibitor 3-bromopyruvate (3-BP) blocks the amino terminus of 4-OT to Edman degradation and results in the disappearance of the 15N resonance of Pro-1 (delta = 49.2 ppm at pH 6.40 and 42 de …
In support of this proposed role, we have found that the active-site-directed irreversible inhibitor 3-bromopyruvate (3-BP) blocks the amino …