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Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location.
Goedhart J, van Unen J, Adjobo-Hermans MJ, Gadella TW Jr. Goedhart J, et al. Sci Rep. 2013;3:2284. doi: 10.1038/srep02284. Sci Rep. 2013. PMID: 23884432 Free PMC article.
Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching.
Van Munster EB, Kremers GJ, Adjobo-Hermans MJ, Gadella TW Jr. Van Munster EB, et al. J Microsc. 2005 Jun;218(Pt 3):253-62. doi: 10.1111/j.1365-2818.2005.01483.x. J Microsc. 2005. PMID: 15958019
Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation.
Adjobo-Hermans MJ, Goedhart J, Gadella TW Jr. Adjobo-Hermans MJ, et al. J Cell Sci. 2006 Dec 15;119(Pt 24):5087-97. doi: 10.1242/jcs.03284. J Cell Sci. 2006. PMID: 17158913
Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.
Goedhart J, Vermeer JE, Adjobo-Hermans MJ, van Weeren L, Gadella TW Jr. Goedhart J, et al. PLoS One. 2007 Oct 10;2(10):e1011. doi: 10.1371/journal.pone.0001011. PLoS One. 2007. PMID: 17925859 Free PMC article.
Regulation of PLCbeta1a membrane anchoring by its substrate phosphatidylinositol (4,5)-bisphosphate.
Adjobo-Hermans MJ, Goedhart J, Gadella TW Jr. Adjobo-Hermans MJ, et al. J Cell Sci. 2008 Nov 15;121(Pt 22):3770-7. doi: 10.1242/jcs.029785. Epub 2008 Oct 28. J Cell Sci. 2008. PMID: 18957514
Real-time visualization of heterotrimeric G protein Gq activation in living cells.
Adjobo-Hermans MJ, Goedhart J, van Weeren L, Nijmeijer S, Manders EM, Offermanns S, Gadella TW Jr. Adjobo-Hermans MJ, et al. BMC Biol. 2011 May 27;9:32. doi: 10.1186/1741-7007-9-32. BMC Biol. 2011. PMID: 21619590 Free PMC article.
Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.
Goedhart J, van Weeren L, Adjobo-Hermans MJ, Elzenaar I, Hink MA, Gadella TW Jr. Goedhart J, et al. PLoS One. 2011;6(11):e27321. doi: 10.1371/journal.pone.0027321. Epub 2011 Nov 17. PLoS One. 2011. PMID: 22114669 Free PMC article.
Analyzing the homeostasis of signaling proteins by a combination of Western blot and fluorescence correlation spectroscopy.
Chung YD, Sinzinger MD, Bovee-Geurts P, Krause M, Dinkla S, Joosten I, Koopman WJ, Adjobo-Hermans MJ, Brock R. Chung YD, et al. Biophys J. 2011 Dec 7;101(11):2807-15. doi: 10.1016/j.bpj.2011.09.058. Biophys J. 2011. PMID: 22261070 Free PMC article.
PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq.
Adjobo-Hermans MJ, Crosby KC, Putyrski M, Bhageloe A, van Weeren L, Schultz C, Goedhart J, Gadella TW Jr. Adjobo-Hermans MJ, et al. Cell Signal. 2013 Jan;25(1):255-63. doi: 10.1016/j.cellsig.2012.09.022. Epub 2012 Sep 21. Cell Signal. 2013. PMID: 23006664
Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane.
Crosby KC, Postma M, Hink MA, Zeelenberg CH, Adjobo-Hermans MJ, Gadella TW. Crosby KC, et al. Biophys J. 2013 May 7;104(9):1875-85. doi: 10.1016/j.bpj.2013.02.057. Biophys J. 2013. PMID: 23663830 Free PMC article.
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