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Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation.
Reyes-Duarte D, Polaina J, López-Cortés N, Alcalde M, Plou FJ, Elborough K, Ballesteros A, Timmis KN, Golyshin PN, Ferrer M. Reyes-Duarte D, et al. Among authors: alcalde m. Angew Chem Int Ed Engl. 2005 Nov 25;44(46):7553-7. doi: 10.1002/anie.200502461. Angew Chem Int Ed Engl. 2005. PMID: 16254934 No abstract available.
Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide.
Zumárraga M, Vaz Domínguez C, Camarero S, Shleev S, Polaina J, Martínez-Arias A, Ferrer M, De Lacey AL, Fernández VM, Ballesteros A, Plou FJ, Alcalde M. Zumárraga M, et al. Among authors: alcalde m. Comb Chem High Throughput Screen. 2008 Dec;11(10):807-16. doi: 10.2174/138620708786734235. Comb Chem High Throughput Screen. 2008. PMID: 19075602
Laboratory evolution of high-redox potential laccases.
Maté D, García-Burgos C, García-Ruiz E, Ballesteros AO, Camarero S, Alcalde M. Maté D, et al. Among authors: alcalde m. Chem Biol. 2010 Sep 24;17(9):1030-41. doi: 10.1016/j.chembiol.2010.07.010. Chem Biol. 2010. PMID: 20851352
266 results