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Sputum phagocytes from healthy individuals are functional and activated: a flow cytometric comparison with cells in bronchoalveolar lavage and peripheral blood.
Alexis N, Soukup J, Ghio A, Becker S. Alexis N, et al. Clin Immunol. 2000 Oct;97(1):21-32. doi: 10.1006/clim.2000.4911. Clin Immunol. 2000. PMID: 10998314
Using flow cytometry, we investigated whether sputum phagocytes demonstrate phenotypes indicative of increased functional activation and inflammation compared to phagocytes from the alveolar airways and peripheral blood (PB) in healthy subjects (N = 17). ...
Using flow cytometry, we investigated whether sputum phagocytes demonstrate phenotypes indicative of increased functional activation and inf …
Cyclooxygenase metabolites play a different role in ozone-induced pulmonary function decline in asthmatics compared to normals.
Alexis N, Urch B, Tarlo S, Corey P, Pengelly D, O'Byrne P, Silverman F. Alexis N, et al. Inhal Toxicol. 2000 Dec;12(12):1205-24. doi: 10.1080/08958370050198548. Inhal Toxicol. 2000. PMID: 11114789
Thirteen (n = 13) Asth and nine (n = 9) Nm volunteers were pretreated with indomethacin or placebo (3 days, 75 mg/day), then exposed for 2 h to 400 ppb ozone or clean air while performing mild intermittent exercise (Vi(min) = 30 L/min.). ...
Thirteen (n = 13) Asth and nine (n = 9) Nm volunteers were pretreated with indomethacin or placebo (3 days, 75 mg/day), then e …
CD14-dependent airway neutrophil response to inhaled LPS: role of atopy.
Alexis N, Eldridge M, Reed W, Bromberg P, Peden DB. Alexis N, et al. J Allergy Clin Immunol. 2001 Jan;107(1):31-5. doi: 10.1067/mai.2001.111594. J Allergy Clin Immunol. 2001. PMID: 11149987
Association between airway hyperreactivity and bronchial macrophage dysfunction in individuals with mild asthma.
Alexis NE, Soukup J, Nierkens S, Becker S. Alexis NE, et al. Am J Physiol Lung Cell Mol Physiol. 2001 Feb;280(2):L369-75. doi: 10.1152/ajplung.2001.280.2.L369. Am J Physiol Lung Cell Mol Physiol. 2001. PMID: 11159017 Clinical Trial.
BMs obtained by induced sputum from mild asthmatics (n = 20) and healthy individuals (n = 20) were analyzed using flow cytometry for CD16, CD64, CD11b, CD14, and human leukocyte antigen-DR expression, phagocytosis of IgG opsonized yeast, and oxidant production. ...
BMs obtained by induced sputum from mild asthmatics (n = 20) and healthy individuals (n = 20) were analyzed using flow cytomet …
Induced sputum derives from the central airways: confirmation using a radiolabeled aerosol bolus delivery technique.
Alexis NE, Hu SC, Zeman K, Alter T, Bennett WD. Alexis NE, et al. Am J Respir Crit Care Med. 2001 Nov 15;164(10 Pt 1):1964-70. doi: 10.1164/ajrccm.164.10.2104051. Am J Respir Crit Care Med. 2001. PMID: 11734453
Iron-binding and storage proteins in sputum.
Alexis NE, Richards JH, Carter JD, Ghio AJ. Alexis NE, et al. Inhal Toxicol. 2002 Apr;14(4):387-400. doi: 10.1080/08958370252871014. Inhal Toxicol. 2002. PMID: 12028811
Seventeen (n = 17) healthy, nonsmoking volunteers underwent sputum induction and fiber-optic bronchoscopy with lavage in order to measure and compare the levels of iron and iron-binding and storage proteins in BAL, bronchial lavage (BL), and IS. ...
Seventeen (n = 17) healthy, nonsmoking volunteers underwent sputum induction and fiber-optic bronchoscopy with lavage in order to mea …
IL-4 induces IL-6 and signs of allergic-type inflammation in the nasal airways of nonallergic individuals.
Alexis N, Griffith K, Almond M, Peden DB. Alexis N, et al. Clin Immunol. 2002 Sep;104(3):217-20. doi: 10.1006/clim.2002.5236. Clin Immunol. 2002. PMID: 12217330
Ten (N = 10) healthy subjects received nasal IL-4 (10 microg) or saline (0.9%) challenges on separate occasions. Nasal lavage was obtained before and 24 h after nasal challenges. ...
Ten (N = 10) healthy subjects received nasal IL-4 (10 microg) or saline (0.9%) challenges on separate occasions. Nasal lavage was obt …
Effect of inhaled endotoxin on airway and circulating inflammatory cell phagocytosis and CD11b expression in atopic asthmatic subjects.
Alexis NE, Eldridge MW, Peden DB. Alexis NE, et al. J Allergy Clin Immunol. 2003 Aug;112(2):353-61. doi: 10.1067/mai.2003.1651. J Allergy Clin Immunol. 2003. PMID: 12897742
Assessment of phagocytosis and cell-surface phenotype (CD11b, CD14, and CD64) was performed on phagocytes obtained from sputum (n = 7) and blood samples (n = 10). ...
Assessment of phagocytosis and cell-surface phenotype (CD11b, CD14, and CD64) was performed on phagocytes obtained from sputum (n = 7 …
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