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Biosynthesis of the Novel Macrolide Antibiotic Anthracimycin.
Alt S, Wilkinson B. Alt S, et al. ACS Chem Biol. 2015 Nov 20;10(11):2468-79. doi: 10.1021/acschembio.5b00525. Epub 2015 Sep 8. ACS Chem Biol. 2015. PMID: 26349074
John's Island, Singapore. The 53 253 bps atc locus includes a trans-acyltransferase (trans-AT) polyketide synthase (PKS), and heterologous expression in Streptomyces coelicolor resulted in anthracimycin production. ...
John's Island, Singapore. The 53 253 bps atc locus includes a trans-acyltransferase (trans-AT) polyketide synthase (PKS), and heterol …
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters.
Wlodek A, Kendrew SG, Coates NJ, Hold A, Pogwizd J, Rudder S, Sheehan LS, Higginbotham SJ, Stanley-Smith AE, Warneck T, Nur-E-Alam M, Radzom M, Martin CJ, Overvoorde L, Samborskyy M, Alt S, Heine D, Carter GT, Graziani EI, Koehn FE, McDonald L, Alanine A, Rodríguez Sarmiento RM, Chao SK, Ratni H, Steward L, Norville IH, Sarkar-Tyson M, Moss SJ, Leadlay PF, Wilkinson B, Gregory MA. Wlodek A, et al. Among authors: alt s. Nat Commun. 2017 Oct 31;8(1):1206. doi: 10.1038/s41467-017-01344-3. Nat Commun. 2017. PMID: 29089518 Free PMC article.
Synchronous intramolecular cycloadditions of the polyene macrolactam polyketide heronamide C.
Booth TJ, Alt S, Capon RJ, Wilkinson B. Booth TJ, et al. Among authors: alt s. Chem Commun (Camb). 2016 May 11;52(38):6383-6. doi: 10.1039/c6cc01930g. Epub 2016 Apr 19. Chem Commun (Camb). 2016. PMID: 27091090
Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products.
Gomez-Escribano JP, Alt S, Bibb MJ. Gomez-Escribano JP, et al. Among authors: alt s. Mar Drugs. 2016 Apr 13;14(4):78. doi: 10.3390/md14040078. Mar Drugs. 2016. PMID: 27089350 Free PMC article. Review.
An artificial pathway to 3,4-dihydroxybenzoic acid allows generation of new aminocoumarin antibiotic recognized by catechol transporters of E. coli.
Alt S, Burkard N, Kulik A, Grond S, Heide L. Alt S, et al. Chem Biol. 2011 Mar 25;18(3):304-13. doi: 10.1016/j.chembiol.2010.12.016. Chem Biol. 2011. PMID: 21439475
Capturing linear intermediates and C-terminal variants during maturation of the thiopeptide GE2270.
Tocchetti A, Maffioli S, Iorio M, Alt S, Mazzei E, Brunati C, Sosio M, Donadio S. Tocchetti A, et al. Among authors: alt s. Chem Biol. 2013 Aug 22;20(8):1067-77. doi: 10.1016/j.chembiol.2013.07.005. Epub 2013 Aug 8. Chem Biol. 2013. PMID: 23932526
Inhibition of DNA gyrase and DNA topoisomerase IV of Staphylococcus aureus and Escherichia coli by aminocoumarin antibiotics.
Alt S, Mitchenall LA, Maxwell A, Heide L. Alt S, et al. J Antimicrob Chemother. 2011 Sep;66(9):2061-9. doi: 10.1093/jac/dkr247. Epub 2011 Jun 21. J Antimicrob Chemother. 2011. PMID: 21693461
Both subunits of S. aureus topoisomerase IV were purified as His-Tag proteins in E. coli. The MIC was tested in vivo for the control organisms E. coli ATCC 25922 and S. aureus ATCC 29213. ...Potassium glutamate is essential for the activity of S. aureus gyras …
Both subunits of S. aureus topoisomerase IV were purified as His-Tag proteins in E. coli. The MIC was tested in vivo for the control …
Biosynthesis of clorobiocin: investigation of the transfer and methylation of the pyrrolyl-2-carboxyl moiety.
Anderle C, Alt S, Gulder T, Bringmann G, Kammerer B, Gust B, Heide L. Anderle C, et al. Among authors: alt s. Arch Microbiol. 2007 Mar;187(3):227-37. doi: 10.1007/s00203-006-0190-9. Epub 2006 Nov 25. Arch Microbiol. 2007. PMID: 17308937
Toward Single-Peak Dalbavancin Analogs through Biology and Chemistry.
Alt S, Bernasconi A, Sosio M, Brunati C, Donadio S, Maffioli SI. Alt S, et al. ACS Chem Biol. 2019 Mar 15;14(3):356-360. doi: 10.1021/acschembio.9b00050. Epub 2019 Mar 7. ACS Chem Biol. 2019. PMID: 30830742
A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete.
Tocchetti A, Bordoni R, Gallo G, Petiti L, Corti G, Alt S, Cruz JC, Salzano AM, Scaloni A, Puglia AM, De Bellis G, Peano C, Donadio S, Sosio M. Tocchetti A, et al. Among authors: alt s. PLoS One. 2015 Jul 24;10(7):e0133705. doi: 10.1371/journal.pone.0133705. eCollection 2015. PLoS One. 2015. PMID: 26207753 Free PMC article.
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