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Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli.
Amann E, Ochs B, Abel KJ. Amann E, et al. Gene. 1988 Sep 30;69(2):301-15. doi: 10.1016/0378-1119(88)90440-4. Gene. 1988. PMID: 3069586
Based on pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], the vectors carry a strong hybrid trp/lac promoter, the lacZ ribosome-binding site (RBS), the multiple cloning site of pUC18 and the rrnB transcription terminators. ...
Based on pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], the vectors carry a strong hybrid trp/lac promoter, the lacZ ribosome- …
Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.
Bagdasarian MM, Amann E, Lurz R, Rückert B, Bagdasarian M. Bagdasarian MM, et al. Gene. 1983 Dec;26(2-3):273-82. doi: 10.1016/0378-1119(83)90197-x. Gene. 1983. PMID: 6323265
Regulated overproduction of APH and catechol 2,3-oxygenase can be obtained with the aid of the new vectors in both E. coli and P. putida....
Regulated overproduction of APH and catechol 2,3-oxygenase can be obtained with the aid of the new vectors in both E. coli and P. put …
Plasmid vectors for the regulated, high level expression of eukaryotic genes in Escherichia coli.
Amann E. Amann E. Dev Biol Stand. 1985;59:11-22. Dev Biol Stand. 1985. PMID: 2989046
A series of plasmid vectors has been constructed for the regulated, high level expression of foreign genes in E. coli. The vectors express cloned genes under the control of the tac promoter, which is a hybrid of trp and lac promoter sequences. ...A third type of vector all …
A series of plasmid vectors has been constructed for the regulated, high level expression of foreign genes in E. coli. The vectors ex …
Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD).
Suzuki R, Asami N, Amann E, Wagatsuma M. Suzuki R, et al. Gene. 1995 Jan 23;152(2):257-60. doi: 10.1016/0378-1119(94)00713-3. Gene. 1995. PMID: 7835711
Cloning and expression of the Bacillus subtilis phage SPP1 in E. coli. I. Construction and characterization of lambda/SPP1 hybrids.
Amann EP, Reeve JN, Morelli G, Behrens B, Trautner TA. Amann EP, et al. Mol Gen Genet. 1981;182(2):293-8. doi: 10.1007/BF00269673. Mol Gen Genet. 1981. PMID: 6457235
A region within EcoRI fragment 1 responsible for its incompatibility with replication in E. coli was defined by these experiments....
A region within EcoRI fragment 1 responsible for its incompatibility with replication in E. coli was defined by these experiments....
Cloning and expression of Bacillus subtilis phage SPP1 in E. coli. II. Expression of lambda/SPP1 hybrid phages in E. coli minicells.
Amann EP, Reeve JN. Amann EP, et al. Mol Gen Genet. 1981;182(2):299-303. doi: 10.1007/BF00269674. Mol Gen Genet. 1981. PMID: 6457236
In the preceding paper (Amann et al. 1981) we described the in vitro construction of hybrids between Escherichia coli phage lambda NM607 imm434 and B. subtilis phage SPP1. ...Infection of E. coli minicells carrying plasmid pGY101, which encodes and expresses the rep …
In the preceding paper (Amann et al. 1981) we described the in vitro construction of hybrids between Escherichia coli phage lambda NM …
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