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An integrated workflow for extraction and solubilization of intermediate filaments from colorectal biopsies for proteomic analysis.
Majumdar D, Rosser R, Havard S, Lobo AJ, Wright PC, Evans CA, Corfe BM. Majumdar D, et al. Electrophoresis. 2012 Jul;33(13):1967-74. doi: 10.1002/elps.201100662. Electrophoresis. 2012. PMID: 22806461
The extraction of cytoskeletal proteins was initially evaluated using MCF-7 cancer cell lines using a published, differential detergent solubilization protocol. IF proteins were extracted from colonic biopsies using a combination of homogenization and sonicat …
The extraction of cytoskeletal proteins was initially evaluated using MCF-7 cancer cell lines using a published, differential deterge …
Coacervation of Lipid Bilayer in Natural Cell Membranes for Extraction, Fractionation, and Enrichment of Proteins in Proteomics Studies.
Koolivand A, Azizi M, O'Brien A, Khaledi MG. Koolivand A, et al. J Proteome Res. 2019 Apr 5;18(4):1595-1606. doi: 10.1021/acs.jproteome.8b00857. Epub 2019 Mar 13. J Proteome Res. 2019. PMID: 30810315
This is the first report where hexafluoroisopropanol (HFIP) was used to induce the coacervation of lipid components in natural cell membranes that would concomitantly result in solubilization, extraction, and enrichment of hydrophobic proteins (e.g., integral
This is the first report where hexafluoroisopropanol (HFIP) was used to induce the coacervation of lipid components in natural cell membrane …
Imaging of compositional gradients during in situ emulsification using X-ray micro-tomography.
Unsal E, Rücker M, Berg S, Bartels WB, Bonnin A. Unsal E, et al. J Colloid Interface Sci. 2019 Aug 15;550:159-169. doi: 10.1016/j.jcis.2019.04.068. Epub 2019 Apr 23. J Colloid Interface Sci. 2019. PMID: 31071522
In this study, a flow unit was integrated in a synchrotron beamline-based fast X-ray computed micro-tomography set-up. Non-destructive three-dimensional visualization of multi phase flow inside a porous rock at flow conditions became viable. ...The sample was continuously …
In this study, a flow unit was integrated in a synchrotron beamline-based fast X-ray computed micro-tomography set-up. Non-destructiv …
Cell wall enrichment unveils proteomic changes in the cell wall during treatment of Mycobacterium smegmatis with sub-lethal concentrations of rifampicin.
Hermann C, Giddey AD, Nel AJM, Soares NC, Blackburn JM. Hermann C, et al. J Proteomics. 2019 Jan 16;191:166-179. doi: 10.1016/j.jprot.2018.02.019. Epub 2018 Feb 18. J Proteomics. 2019. PMID: 29466714
The cell wall was subjected to differential centrifugation, differential detergent solubilisation and phase separation to yield the genuine cell wall proteome. ...We then provide a data analysis workflow that enables the removal of likely cytosolic contaminants in t …
The cell wall was subjected to differential centrifugation, differential detergent solubilisation and phase separation to yield the g …
Deep amino acid sequencing of native brain GABAA receptors using high-resolution mass spectrometry.
Chen ZW, Fuchs K, Sieghart W, Townsend RR, Evers AS. Chen ZW, et al. Mol Cell Proteomics. 2012 Jan;11(1):M111.011445. doi: 10.1074/mcp.M111.011445. Mol Cell Proteomics. 2012. PMID: 22338125 Free PMC article.
Mass spectrometric sequencing of low abundance, integral membrane proteins, particularly the transmembrane domains, presents challenges that span the multiple phases of sample preparation including solubilization, purification, enzymatic digestion, peptide extrac
Mass spectrometric sequencing of low abundance, integral membrane proteins, particularly the transmembrane domains, presents challeng …
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