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An optimized fluorescent probe for visualizing glutamate neurotransmission.
Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter ER, Demb JB, Gan WB, Hires SA, Looger LL. Marvin JS, et al. Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 2013 Jan 13. Nat Methods. 2013. PMID: 23314171 Free PMC article.
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visuali
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appro …
High-throughput development of a hybrid-type fluorescent glutamate sensor for analysis of synaptic transmission.
Takikawa K, Asanuma D, Namiki S, Sakamoto H, Ariyoshi T, Kimpara N, Hirose K. Takikawa K, et al. Angew Chem Int Ed Engl. 2014 Dec 1;53(49):13439-43. doi: 10.1002/anie.201407181. Epub 2014 Oct 8. Angew Chem Int Ed Engl. 2014. PMID: 25297726
Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high-throughput screening system, designated hybrid-type fluorescence indicator development (HyFInD), to identify optimal position-specific fluorophore labeling i
Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high-throughput screening sys
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