An optimized fluorescent probe for visualizing glutamate neurotransmission.
Nat Methods. 2013.
PMID: 23314171
Free PMC article.
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An optimized fluorescent probe for visualizing glutamate neurotransmission.
Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter ER, Demb JB, Gan WB, Hires SA, Looger LL.
Marvin JS, et al.
Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 2013 Jan 13.
Nat Methods. 2013.
PMID: 23314171
Free PMC article.
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visuali …
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appro …
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High-throughput development of a hybrid-type fluorescent glutamate sensor for analysis of synaptic transmission.
Takikawa K, Asanuma D, Namiki S, Sakamoto H, Ariyoshi T, Kimpara N, Hirose K.
Takikawa K, et al.
Angew Chem Int Ed Engl. 2014 Dec 1;53(49):13439-43. doi: 10.1002/anie.201407181. Epub 2014 Oct 8.
Angew Chem Int Ed Engl. 2014.
PMID: 25297726
We screened sensors for glutamate among hybrid molecules obtained by the reaction of four cysteine-reactive fluorescence probes with a set of cysteine-scanning mutants of the 274 amino acid S1S2 domain of AMPA-type glutamate receptor GluA2 subunit. HyFInD ide …
We screened sensors for glutamate among hybrid molecules obtained by the reaction of four cysteine-reactive fluorescence probes with …
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