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Phosphopeptide analysis by matrix-assisted laser desorption time-of-flight mass spectrometry.
Annan RS, Carr SA. Annan RS, et al. Anal Chem. 1996 Oct 1;68(19):3413-21. doi: 10.1021/ac960221g. Anal Chem. 1996. PMID: 8843139
Mapping posttranslational modifications of proteins by MS-based selective detection: application to phosphoproteomics.
Carr SA, Annan RS, Huddleston MJ. Carr SA, et al. Among authors: annan rs. Methods Enzymol. 2005;405:82-115. doi: 10.1016/S0076-6879(05)05005-6. Methods Enzymol. 2005. PMID: 16413312 Review.
Improved sensitivity for phosphopeptide mapping using capillary column HPLC and microionspray mass spectrometry: comparative phosphorylation site mapping from gel-derived proteins.
Zappacosta F, Huddleston MJ, Karcher RL, Gelfand VI, Carr SA, Annan RS. Zappacosta F, et al. Among authors: annan rs. Anal Chem. 2002 Jul 1;74(13):3221-31. doi: 10.1021/ac025538x. Anal Chem. 2002. PMID: 12141686
Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast.
Loughrey Chen S, Huddleston MJ, Shou W, Deshaies RJ, Annan RS, Carr SA. Loughrey Chen S, et al. Among authors: annan rs. Mol Cell Proteomics. 2002 Mar;1(3):186-96. doi: 10.1074/mcp.m100032-mcp200. Mol Cell Proteomics. 2002. PMID: 12096118
Protein posttranslational modifications: phosphorylation site analysis using mass spectrometry.
Annan RS, Zappacosta F. Annan RS, et al. Methods Biochem Anal. 2005;45:85-106. Methods Biochem Anal. 2005. PMID: 19235292 Review. No abstract available.
Hydrophilic interaction chromatography reduces the complexity of the phosphoproteome and improves global phosphopeptide isolation and detection.
McNulty DE, Annan RS. McNulty DE, et al. Among authors: annan rs. Mol Cell Proteomics. 2008 May;7(5):971-80. doi: 10.1074/mcp.M700543-MCP200. Epub 2008 Jan 22. Mol Cell Proteomics. 2008. PMID: 18212344
A quantitative results-driven approach to analyzing multisite protein phosphorylation: the phosphate-dependent phosphorylation profile of the transcription factor Pho4.
Zappacosta F, Collingwood TS, Huddleston MJ, Annan RS. Zappacosta F, et al. Among authors: annan rs. Mol Cell Proteomics. 2006 Nov;5(11):2019-30. doi: 10.1074/mcp.M600238-MCP200. Epub 2006 Jul 6. Mol Cell Proteomics. 2006. PMID: 16825185
N-terminal isotope tagging strategy for quantitative proteomics: results-driven analysis of protein abundance changes.
Zappacosta F, Annan RS. Zappacosta F, et al. Among authors: annan rs. Anal Chem. 2004 Nov 15;76(22):6618-27. doi: 10.1021/ac049169b. Anal Chem. 2004. PMID: 15538785
N-Terminal peptide labeling strategy for incorporation of isotopic tags: a method for the determination of site-specific absolute phosphorylation stoichiometry.
Zhang X, Jin QK, Carr SA, Annan RS. Zhang X, et al. Among authors: annan rs. Rapid Commun Mass Spectrom. 2002;16(24):2325-32. doi: 10.1002/rcm.864. Rapid Commun Mass Spectrom. 2002. PMID: 12478578
Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach.
Zappacosta F, Huddleston MJ, Annan RS. Zappacosta F, et al. Among authors: annan rs. Methods Mol Biol. 2004;284:91-110. doi: 10.1385/1-59259-816-1:091. Methods Mol Biol. 2004. PMID: 15173611
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