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Role of mitochondrial and cytoplasmic serine hydroxymethyltransferase isozymes in de novo purine synthesis in Saccharomyces cerevisiae.
Kastanos EK, Woldman YY, Appling DR. Kastanos EK, et al. Among authors: appling dr. Biochemistry. 1997 Dec 2;36(48):14956-64. doi: 10.1021/bi971610n. Biochemistry. 1997. PMID: 9398220
13C NMR detection of folate-mediated serine and glycine synthesis in vivo in Saccharomyces cerevisiae.
Pasternack LB, Laude DA Jr, Appling DR. Pasternack LB, et al. Among authors: appling dr. Biochemistry. 1992 Sep 22;31(37):8713-9. doi: 10.1021/bi00152a005. Biochemistry. 1992. PMID: 1390656
Compartmentation of folate-mediated one-carbon metabolism in eukaryotes.
Appling DR. Appling DR. FASEB J. 1991 Sep;5(12):2645-51. doi: 10.1096/fasebj.5.12.1916088. FASEB J. 1991. PMID: 1916088 Review.
Rat C1-tetrahydrofolate synthase. cDNA isolation, tissue-specific levels of the mRNA, and expression of the protein in yeast.
Thigpen AE, West MG, Appling DR. Thigpen AE, et al. Among authors: appling dr. J Biol Chem. 1990 May 15;265(14):7907-13. J Biol Chem. 1990. PMID: 2186031
Isolation and characterization of a novel eukaryotic monofunctional NAD(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase.
Barlowe CK, Appling DR. Barlowe CK, et al. Among authors: appling dr. Biochemistry. 1990 Jul 31;29(30):7089-94. doi: 10.1021/bi00482a020. Biochemistry. 1990. PMID: 2223762
Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme.
Barlowe CK, Appling DR. Barlowe CK, et al. Among authors: appling dr. Mol Cell Biol. 1990 Nov;10(11):5679-87. doi: 10.1128/mcb.10.11.5679. Mol Cell Biol. 1990. PMID: 2233711 Free PMC article.
Appling, Biochemistry 29:7089-7094, 1990). We propose that the characteristics of strains expressing full-length but catalytically inactive C1-THF synthase could result from the formation of a purine-synthesizing multienzyme complex involving the structurally unchanged C1-
Appling, Biochemistry 29:7089-7094, 1990). We propose that the characteristics of strains expressing full-length but catalytically in
Purification, immunoassay, and tissue distribution of rat C1-tetrahydrofolate synthase.
Cheek WD, Appling DR. Cheek WD, et al. Among authors: appling dr. Arch Biochem Biophys. 1989 May 1;270(2):504-12. doi: 10.1016/0003-9861(89)90532-8. Arch Biochem Biophys. 1989. PMID: 2468308
In vitro evidence for the involvement of mitochondrial folate metabolism in the supply of cytoplasmic one-carbon units.
Barlowe CK, Appling DR. Barlowe CK, et al. Among authors: appling dr. Biofactors. 1988 Jul;1(2):171-6. Biofactors. 1988. PMID: 2475123
Site-directed mutagenesis of yeast C1-tetrahydrofolate synthase: analysis of an overlapping active site in a multifunctional enzyme.
Barlowe CK, Williams ME, Rabinowitz JC, Appling DR. Barlowe CK, et al. Among authors: appling dr. Biochemistry. 1989 Mar 7;28(5):2099-106. doi: 10.1021/bi00431a020. Biochemistry. 1989. PMID: 2541774
Previous chemical modification studies on C1-THF synthase from the yeast Saccharomyces cerevisiae indicated at least two cysteinyl residues involved in the dehydrogenase/cyclohydrolase reactions [Appling, D. ...
Previous chemical modification studies on C1-THF synthase from the yeast Saccharomyces cerevisiae indicated at least two cysteinyl residues …
A general method for generation and analysis of defined mutations in enzymes involved in a tetrahydrofolate-interconversion pathway.
Barlowe CK, Appling DR. Barlowe CK, et al. Among authors: appling dr. Biofactors. 1989 Mar;2(1):57-63. Biofactors. 1989. PMID: 2679653
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