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Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells.
Lambrus BG, Moyer TC, Holland AJ. Lambrus BG, et al. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. Methods Cell Biol. 2018. PMID: 29804665
Furthermore, these methods are usually not reversible. Recent advances to achieve protein depletion function by inducibly trafficking proteins of interest to an endogenous E3 ubiquitin ligase complex to promote ubiquitination and subsequent degradation
Furthermore, these methods are usually not reversible. Recent advances to achieve protein depletion function by inducibly traf …
The auxin-inducible degradation system enables conditional PERIOD protein depletion in the nervous system of Drosophila melanogaster.
Chen W, Werdann M, Zhang Y. Chen W, et al. FEBS J. 2018 Dec;285(23):4378-4393. doi: 10.1111/febs.14677. Epub 2018 Oct 25. FEBS J. 2018. PMID: 30321477 Free PMC article.
Tools that allow inducible and reversible depletion of target proteins are critical for biological studies. The plant-derived auxin-inducible degradation system (AID) enables the degradation of target proteins tagged with th …
Tools that allow inducible and reversible depletion of target proteins are critical for biological studies. The plant-derived …
A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.
Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T. Nishimura K, et al. Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. Nucleic Acids Res. 2020. PMID: 32941625 Free PMC article.
The auxin-inducible degron (AID) system enables rapid depletion of target proteins within the cell by applying the natural auxin IAA. The AID system is useful for investigating the physiological functions of essential pro
The auxin-inducible degron (AID) system enables rapid depletion of target proteins within the cell …