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The Human Protein Atlas as a proteomic resource for biomarker discovery.
Pontén F, Schwenk JM, Asplund A, Edqvist PH. Pontén F, et al. J Intern Med. 2011 Nov;270(5):428-46. doi: 10.1111/j.1365-2796.2011.02427.x. Epub 2011 Aug 3. J Intern Med. 2011. PMID: 21752111 Review.
Antibody-based proteomics provides a strategy for the systematic generation of antibodies against all human proteins to combine with protein profiling in tissues and cells using tissue microarrays, immunohistochemistry and immunofluorescence. The Human Protein Atlas projec …
Antibody-based proteomics provides a strategy for the systematic generation of antibodies against all human proteins to combine with …
Pronounced lowering of serum levels of lipoprotein Lp(a) in hyperlipidaemic subjects treated with nicotinic acid.
Carlson LA, Hamsten A, Asplund A. Carlson LA, et al. J Intern Med. 1989 Oct;226(4):271-6. doi: 10.1111/j.1365-2796.1989.tb01393.x. J Intern Med. 1989. PMID: 2530298
Nicotinic acid significantly reduced the serum levels of Lp(a) in the whole patient group. Linear regression analysis showed a strong negative relationship between the percentage reduction of Lp(a) and the serum triglyceride level before treatment (r = -0.78) …
Nicotinic acid significantly reduced the serum levels of Lp(a) in the whole patient group. Linear regression analysis showed a
Clonality analysis of synchronous lesions of cervical carcinoma based on X chromosome inactivation polymorphism, human papillomavirus type 16 genome mutations, and loss of heterozygosity.
Hu X, Pang T, Asplund A, Pontén J, Nistér M. Hu X, et al. J Exp Med. 2002 Apr 1;195(7):845-54. doi: 10.1084/jem.20011564. J Exp Med. 2002. PMID: 11927629 Free PMC article.
To understand the causes, origin, and model of progression of cervical carcinoma, we assessed the clonality of a case with multiple synchronous lesions by analyzing X chromosome inactivation polymorphism, human papillomavirus type 16 (HPV16) sequence variation/mutations, a …
To understand the causes, origin, and model of progression of cervical carcinoma, we assessed the clonality of a case with multiple s …
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
Agaton C, Galli J, Höidén Guthenberg I, Janzon L, Hansson M, Asplund A, Brundell E, Lindberg S, Ruthberg I, Wester K, Wurtz D, Höög C, Lundeberg J, Ståhl S, Pontén F, Uhlén M. Agaton C, et al. Mol Cell Proteomics. 2003 Jun;2(6):405-14. doi: 10.1074/mcp.M300022-MCP200. Epub 2003 Jun 9. Mol Cell Proteomics. 2003. PMID: 12796447
Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distrib …
Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative …
Mutation spectra of epidermal p53 clones adjacent to basal cell carcinoma and squamous cell carcinoma.
Bäckvall H, Strömberg S, Gustafsson A, Asplund A, Sivertsson A, Lundeberg J, Ponten F. Bäckvall H, et al. Exp Dermatol. 2004 Oct;13(10):643-50. doi: 10.1111/j.0906-6705.2004.00211.x. Exp Dermatol. 2004. PMID: 15447725
Such epidermal p53 clones are common in chronically sun-exposed skin and have been suggested to play a role in skin cancer development. ...Genetic analysis was performed using a multiplex/nested polymerase chain reaction followed by direct DNA sequencing of p53 exon …
Such epidermal p53 clones are common in chronically sun-exposed skin and have been suggested to play a role in skin cancer developmen …
Genetic tumor archeology: microdissection and genetic heterogeneity in squamous and basal cell carcinoma.
Bäckvall H, Asplund A, Gustafsson A, Sivertsson A, Lundeberg J, Ponten F. Bäckvall H, et al. Mutat Res. 2005 Apr 1;571(1-2):65-79. doi: 10.1016/j.mrfmmm.2004.10.011. Mutat Res. 2005. PMID: 15748639 Review.
A high frequency of epidermal p53 clones has been detected in chronically sun-exposed skin. The abundance of clones containing p53 mutated keratinocytes adjacent to basal cell (BCC) and squamous cell carcinoma (SCC) suggests a role in human skin carcinogenesis. Stud
A high frequency of epidermal p53 clones has been detected in chronically sun-exposed skin. The abundance of clones containing p53 mu
Genetic mosaicism in basal cell carcinoma.
Asplund A, Sivertsson A, Bäckvall H, Ahmadian A, Lundeberg J, Ponten F. Asplund A, et al. Exp Dermatol. 2005 Aug;14(8):593-600. doi: 10.1111/j.0906-6705.2005.00333.x. Exp Dermatol. 2005. PMID: 16026581
Human basal cell cancer (BCC) shows unique growth characteristics, including a virtual inability to metastasize, absence of a precursor stage and lack of tumour progression. The clonal nature of BCC has long been a subject for debate because of the tumour gro …
Human basal cell cancer (BCC) shows unique growth characteristics, including a virtual inability to metastasize, absence of a
A human protein atlas for normal and cancer tissues based on antibody proteomics.
Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F. Uhlén M, et al. Mol Cell Proteomics. 2005 Dec;4(12):1920-32. doi: 10.1074/mcp.M500279-MCP200. Epub 2005 Aug 27. Mol Cell Proteomics. 2005. PMID: 16127175
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. ...Each image has been annotated by a certified pathologist to provide a knowledge …
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic genera …
A high-throughput strategy for protein profiling in cell microarrays using automated image analysis.
Strömberg S, Björklund MG, Asplund C, Sköllermo A, Persson A, Wester K, Kampf C, Nilsson P, Andersson AC, Uhlen M, Kononen J, Ponten F, Asplund A. Strömberg S, et al. Proteomics. 2007 Jun;7(13):2142-50. doi: 10.1002/pmic.200700199. Proteomics. 2007. PMID: 17549799
Here we present a high-throughput strategy combining IHC on a recently developed cell microarray with a novel, automated image-analysis application (TMAx). ...In conclusion, automated analysis of immunohistochemically stained in vitro-cultured cells in a
Here we present a high-throughput strategy combining IHC on a recently developed cell microarray with a novel, automate …
Toward a confocal subcellular atlas of the human proteome.
Barbe L, Lundberg E, Oksvold P, Stenius A, Lewin E, Björling E, Asplund A, Pontén F, Brismar H, Uhlén M, Andersson-Svahn H. Barbe L, et al. Mol Cell Proteomics. 2008 Mar;7(3):499-508. doi: 10.1074/mcp.M700325-MCP200. Epub 2007 Nov 19. Mol Cell Proteomics. 2008. PMID: 18029348
Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. ...The results suggest that this approach might be a valuable tool in conjunction with predictive mod …
Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis usi …
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