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2011 1
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2020 28
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Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution.
Cheng JB, Sedgewick AJ, Finnegan AI, Harirchian P, Lee J, Kwon S, Fassett MS, Golovato J, Gray M, Ghadially R, Liao W, Perez White BE, Mauro TM, Mully T, Kim EA, Sbitany H, Neuhaus IM, Grekin RC, Yu SS, Gray JW, Purdom E, Paus R, Vaske CJ, Benz SC, Song JS, Cho RJ. Cheng JB, et al. Cell Rep. 2018 Oct 23;25(4):871-883. doi: 10.1016/j.celrep.2018.09.006. Cell Rep. 2018. PMID: 30355494 Free PMC article.
However, the global scope and organization of this complex cellular program remain undefined. Here we report single-cell RNA sequencing profiles of 92,889 human epidermal cells from 9 normal and 3 inflamed skin samples. Transcriptomics-derived keratinocyte su …
However, the global scope and organization of this complex cellular program remain undefined. Here we report single-cell RNA s …
Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development.
Su X, Shi Y, Zou X, Lu ZN, Xie G, Yang JYH, Wu CC, Cui XF, He KY, Luo Q, Qu YL, Wang N, Wang L, Han ZG. Su X, et al. BMC Genomics. 2017 Dec 4;18(1):946. doi: 10.1186/s12864-017-4342-x. BMC Genomics. 2017. PMID: 29202695 Free PMC article.
BACKGROUND: The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking. RESULTS: We employed marker- …
BACKGROUND: The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at …
Single-cell RNA-seq reveals different subsets of non-specific cytotoxic cells in teleost.
Niu J, Huang Y, Liu X, Zhang Z, Tang J, Wang B, Lu Y, Cai J, Jian J. Niu J, et al. Genomics. 2020 Nov;112(6):5170-5179. doi: 10.1016/j.ygeno.2020.09.031. Epub 2020 Sep 21. Genomics. 2020. PMID: 32971213
However, the NCC subsets have not been clarified. Thus, we create a comprehensive cell map of ~24,062 head kidney-derived leukocytes from Nile tilapia post poly I:C stimulation using single-cell RNA sequencing (scRNA-seq). Based on cell heteroge …
However, the NCC subsets have not been clarified. Thus, we create a comprehensive cell map of ~24,062 head kidney-derived leukocytes …
Circular RNA circMET drives immunosuppression and anti-PD1 therapy resistance in hepatocellular carcinoma via the miR-30-5p/snail/DPP4 axis.
Huang XY, Zhang PF, Wei CY, Peng R, Lu JC, Gao C, Cai JB, Yang X, Fan J, Ke AW, Zhou J, Shi GM. Huang XY, et al. Mol Cancer. 2020 May 19;19(1):92. doi: 10.1186/s12943-020-01213-6. Mol Cancer. 2020. PMID: 32430013 Free PMC article.
In addition, we used cultured HCC cells to modify circRNA expression and assessed the effects in several cell-based assays as well as gene expression analyses via RNA-seq. Modified cells were implanted into immunocompetent mice to asse
In addition, we used cultured HCC cells to modify circRNA expression and assessed the effects in several cell-based ass …
An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury.
Liu C, Yin Z, Feng T, Zhang M, Zhou Z, Zhou Y. Liu C, et al. J Ethnopharmacol. 2021 Jan 10;264:113364. doi: 10.1016/j.jep.2020.113364. Epub 2020 Sep 9. J Ethnopharmacol. 2021. PMID: 32916233 Free PMC article.
Then, we used compound-target and target-pathway networks to uncover the preventive mechanisms of LJF. Furthermore, we assessed the preventive effects of LJF in an LPS-induced rat model with the RNA-Seq technique to validate the possible molecular mechanisms …
Then, we used compound-target and target-pathway networks to uncover the preventive mechanisms of LJF. Furthermore, we assessed the p …
Sirt6 promotes tumorigenesis and drug resistance of diffuse large B-cell lymphoma by mediating PI3K/Akt signaling.
Yang J, Li Y, Zhang Y, Fang X, Chen N, Zhou X, Wang X. Yang J, et al. J Exp Clin Cancer Res. 2020 Jul 25;39(1):142. doi: 10.1186/s13046-020-01623-w. J Exp Clin Cancer Res. 2020. PMID: 32711549 Free PMC article.
Lentivirus vectors either encoding shSirt6, lvSirt6 or empty lentiviral vector were stably transfected into DLBCL cells. LY1 cell transfected with shSirt6 were performed RNA-sequencing (RNA-seq) analysis, functional enrichment analyses of gene o …
Lentivirus vectors either encoding shSirt6, lvSirt6 or empty lentiviral vector were stably transfected into DLBCL cells. LY1 cell
Mapping macrophage polarization over the myocardial infarction time continuum.
Mouton AJ, DeLeon-Pennell KY, Rivera Gonzalez OJ, Flynn ER, Freeman TC, Saucerman JJ, Garrett MR, Ma Y, Harmancey R, Lindsey ML. Mouton AJ, et al. Basic Res Cardiol. 2018 Jun 4;113(4):26. doi: 10.1007/s00395-018-0686-x. Basic Res Cardiol. 2018. PMID: 29868933 Free PMC article.
We hypothesized that macrophages undergo polarization state changes over the MI time course and assessed macrophage polarization transcriptomic signatures over the first week of MI. ...Day 0, no MI resident cardiac macrophages served as the negative MI control. Whole trans …
We hypothesized that macrophages undergo polarization state changes over the MI time course and assessed macrophage polarization tran …
Mapping the genomic landscape of CRISPR-Cas9 cleavage.
Cameron P, Fuller CK, Donohoue PD, Jones BN, Thompson MS, Carter MM, Gradia S, Vidal B, Garner E, Slorach EM, Lau E, Banh LM, Lied AM, Edwards LS, Settle AH, Capurso D, Llaca V, Deschamps S, Cigan M, Young JK, May AP. Cameron P, et al. Nat Methods. 2017 Jun;14(6):600-606. doi: 10.1038/nmeth.4284. Epub 2017 May 1. Nat Methods. 2017. PMID: 28459459
RNA-guided CRISPR-Cas9 endonucleases are widely used for genome engineering, but our understanding of Cas9 specificity remains incomplete. ...Sites identified at lower concentrations showed a higher propensity for off-target mutations in cells. The list of of
RNA-guided CRISPR-Cas9 endonucleases are widely used for genome engineering, but our understanding of Cas9 specificity remains
Human pluripotent stem cell-derived neural constructs for predicting neural toxicity.
Schwartz MP, Hou Z, Propson NE, Zhang J, Engstrom CJ, Santos Costa V, Jiang P, Nguyen BK, Bolin JM, Daly W, Wang Y, Stewart R, Page CD, Murphy WL, Thomson JA. Schwartz MP, et al. Proc Natl Acad Sci U S A. 2015 Oct 6;112(40):12516-21. doi: 10.1073/pnas.1516645112. Epub 2015 Sep 21. Proc Natl Acad Sci U S A. 2015. PMID: 26392547 Free PMC article.
Here, human embryonic stem (ES) cell-derived neural progenitor cells, endothelial cells, mesenchymal stem cells, and microglia/macrophage precursors were combined on chemically defined polyethylene glycol hydrogels and cultured in serum-free medium to …
Here, human embryonic stem (ES) cell-derived neural progenitor cells, endothelial cells, mesenchymal stem cells, …
Cancer-associated fibroblasts-derived VCAM1 induced by H. pylori infection facilitates tumor invasion in gastric cancer.
Shen J, Zhai J, You Q, Zhang G, He M, Yao X, Shen L. Shen J, et al. Oncogene. 2020 Apr;39(14):2961-2974. doi: 10.1038/s41388-020-1197-4. Epub 2020 Feb 7. Oncogene. 2020. PMID: 32034307
Gene signature of H. pylori-infected human stomach fibroblasts was performed using the RNA-seq analysis. Spheroid cell invasion assay and zebrafish cell line-derived xenograft (zCDX) model were introduced to evaluate tumor invasion induced by CAFs. ... …
Gene signature of H. pylori-infected human stomach fibroblasts was performed using the RNA-seq analysis. Spheroid cell
109 results