Abstract
Within this chapter we outline an A. tumefaciens-mediated transformation method for B. rapa using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation efficiencies are typically achieved in the region of 1% (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / genetics
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Agrobacterium tumefaciens / growth & development
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Brassica / genetics*
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Brassica / growth & development*
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Coculture Techniques
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Cotyledon / genetics
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Cotyledon / growth & development
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Environment, Controlled
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Genetic Engineering / methods*
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Germination
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Plant Shoots / genetics
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Plant Shoots / growth & development
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Sterilization
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Transformation, Genetic