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A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants.
Morales VM, Bäckman A, Bagdasarian M. Morales VM, et al. Gene. 1991 Jan 2;97(1):39-47. doi: 10.1016/0378-1119(91)90007-x. Gene. 1991. PMID: 1847347
A series of controlled expression vectors was constructed based on the wide-host-range plasmid pMMB66EH. Some of these new vectors code for the alpha-peptide of beta-galactosidase and allow the direct screening of recombinant clones by inactivation of alpha-complementation
A series of controlled expression vectors was constructed based on the wide-host-range plasmid pMMB66EH. Some of these new vectors co
Analysis of the V antigen lcrGVH-yopBD operon of Yersinia pseudotuberculosis: evidence for a regulatory role of LcrH and LcrV.
Bergman T, Håkansson S, Forsberg A, Norlander L, Macellaro A, Bäckman A, Bölin I, Wolf-Watz H. Bergman T, et al. J Bacteriol. 1991 Mar;173(5):1607-16. doi: 10.1128/jb.173.5.1607-1616.1991. J Bacteriol. 1991. PMID: 1705541 Free PMC article.
The V antigen operon was recently shown to be involved in the Ca2(+)-regulated negative pathway (A. Forsberg and H. Wolf-Watz, Mol. ...We show here that the V antigen-containing operon of Yersinia pseudotuberculosis is a polycistronic operon having the gene order lc …
The V antigen operon was recently shown to be involved in the Ca2(+)-regulated negative pathway (A. Forsberg and H. Wolf-Watz, Mol. . …
Cloning, expression, and characterization of stratum corneum chymotryptic enzyme. A skin-specific human serine proteinase.
Hansson L, Strömqvist M, Bäckman A, Wallbrandt P, Carlstein A, Egelrud T. Hansson L, et al. J Biol Chem. 1994 Jul 29;269(30):19420-6. J Biol Chem. 1994. PMID: 8034709
The results show that recombinant SCCE is produced with a 22-amino acid residue signal peptide and a propeptide of 7 amino acid residues. ...Recombinant SCCE was found to be heterogenous regarding glycosylation in a manner similar to that of the native enzyme …
The results show that recombinant SCCE is produced with a 22-amino acid residue signal peptide and a propeptide of 7 amino aci …
Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme.
Bäckman A, Strandén P, Brattsand M, Hansson L, Egelrud T. Bäckman A, et al. J Invest Dermatol. 1999 Aug;113(2):152-5. doi: 10.1046/j.1523-1747.1999.00662.x. J Invest Dermatol. 1999. PMID: 10469296
A cDNA obtained by reverse transcription-polymerase chain reaction with total RNA prepared from mouse tails as starting material was cloned, and the expression of the corresponding mRNA studied. ...The murine protein was deduced to have a 21 amino acid signal peptid
A cDNA obtained by reverse transcription-polymerase chain reaction with total RNA prepared from mouse tails as starting material was
Nested PCR optimized for detection of Bordetella pertussis in clinical nasopharyngeal samples.
Bäckman A, Johansson B, Olcén P. Bäckman A, et al. J Clin Microbiol. 1994 Oct;32(10):2544-8. doi: 10.1128/JCM.32.10.2544-2548.1994. J Clin Microbiol. 1994. PMID: 7814495 Free PMC article.
A previously developed single-step PCR assay for the detection of B. pertussis was based on an insertion sequence, IS480, that is present in about 70 to 80 copies in each genome. ...The assay also detected 18 positive aspirates from a total of 196 culture-negative p
A previously developed single-step PCR assay for the detection of B. pertussis was based on an insertion sequence, IS480, that is pre
Amplification of DNA by the polymerase chain reaction for the efficient diagnosis of pertussis.
Olcén P, Bäckman A, Johansson B, Esbjörner E, Törnqvist E, Bygraves J, McPheat WL. Olcén P, et al. Scand J Infect Dis. 1992;24(3):339-45. doi: 10.3109/00365549209061340. Scand J Infect Dis. 1992. PMID: 1509238
In nasopharyngeal aspirates a B. pertussis specific PCR product was visualized in 19/25 culture positive and in 5/50 culture negative children. In conclusion the present PCR assay for B. pertussis can be clinically useful and permit a specific diagnosis within 1 day …
In nasopharyngeal aspirates a B. pertussis specific PCR product was visualized in 19/25 culture positive and in 5/50 culture negative …
Carbohydrate specificity of the Escherichia coli P-pilus papG protein is mediated by its N-terminal part.
Hansson L, Wallbrandt P, Andersson JO, Byström M, Bäckman A, Carlstein A, Enquist K, Lönn H, Otter C, Strömqvist M. Hansson L, et al. Biochim Biophys Acta. 1995 Jun 9;1244(2-3):377-83. doi: 10.1016/0304-4165(95)00028-a. Biochim Biophys Acta. 1995. PMID: 7599158
The results showed that both carbohydrate binding and specificity of the papG adhesin resided in a linear part of the N-terminus of the protein....
The results showed that both carbohydrate binding and specificity of the papG adhesin resided in a linear part of the N-terminus of t …
Evaluation of a PCR method to determine the clinical significance of blood cultures with Staphylococcus epidermidis in patients with hematological malignancies.
Ahlstrand E, Bäckman A, Persson L, Mölling P, Tidefelt U, Söderquist B. Ahlstrand E, et al. APMIS. 2014 Jun;122(6):539-44. doi: 10.1111/apm.12182. Epub 2013 Sep 30. APMIS. 2014. PMID: 24106819
In addition, DNA from S. epidermidis and a selection of other bacterial species were analyzed. Three different sets of criteria were used to classify episodes with positive blood cultures with S. epidermidis as BSIs or contaminations. ...There was no clear association betw …
In addition, DNA from S. epidermidis and a selection of other bacterial species were analyzed. Three different sets of criteria were …
PCR of cerebrospinal fluid for diagnosis of bacterial meningitis during meningococcal epidemics; an example from Sudan.
Issa M, Mölling P, Bäckman A, Unemo M, Sulaiman N, Olcén P. Issa M, et al. Scand J Infect Dis. 2003;35(10):719-23. doi: 10.1080/00365540310016367. Scand J Infect Dis. 2003. PMID: 14606610
A specific PCR for group A meningococci (based on the sacC gene) was positive in 27 of the CSF samples. The results show that PCR technology is a sharp-edged tool for confirmation of a diagnosis of meningococcal meningitis and for obtaining a di
A specific PCR for group A meningococci (based on the sacC gene) was positive in 27 of the CSF samples. The results show that
Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia.
Ohlin A, Bäckman A, Björkqvist M, Mölling P, Jurstrand M, Schollin J. Ohlin A, et al. Acta Paediatr. 2008 Oct;97(10):1376-80. doi: 10.1111/j.1651-2227.2008.00924.x. Epub 2008 Jul 9. Acta Paediatr. 2008. PMID: 18624992
RESULTS: When applied to a material from 50 patients with positive blood culture and 245 patients with negative blood culture, the universal PCR showed a sensitivity of 42% (28-57), a specificity of 95% (92-97), a positive predictive value of 64% (45-8 …
RESULTS: When applied to a material from 50 patients with positive blood culture and 245 patients with negative blood culture, the un …
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