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CRISPR/Cas9 mediated genome engineering in Drosophila.
Bassett A, Liu JL. Bassett A, et al. Methods. 2014 Sep;69(2):128-36. doi: 10.1016/j.ymeth.2014.02.019. Epub 2014 Feb 24. Methods. 2014. PMID: 24576617
Here we describe a simple and efficient technique to generate and detect novel mutations in desired target genes in Drosophila melanogaster. ...
Here we describe a simple and efficient technique to generate and detect novel mutations in desired target genes in Drosophila melano …
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering.
Wu Q, Ferry QRV, Baeumler TA, Michaels YS, Vitsios DM, Habib O, Arnold R, Jiang X, Maio S, Steinkraus BR, Tapia M, Piazza P, Xu N, Holländer GA, Milne TA, Kim JS, Enright AJ, Bassett AR, Fulga TA. Wu Q, et al. Among authors: bassett ar. Nat Commun. 2017 Dec 13;8(1):2109. doi: 10.1038/s41467-017-00686-2. Nat Commun. 2017. PMID: 29235467 Free PMC article.
We use GenERA to survey the entire regulatory landscape of a 3'UTR, and apply it in a multiplex fashion to analyse combinatorial interactions between sets of miRNA response elements (MREs), providing strong evidence for cooperative activity. ...This study provides …
We use GenERA to survey the entire regulatory landscape of a 3'UTR, and apply it in a multiplex fashion to analyse combinatori …
Considerations when investigating lncRNA function in vivo.
Bassett AR, Akhtar A, Barlow DP, Bird AP, Brockdorff N, Duboule D, Ephrussi A, Ferguson-Smith AC, Gingeras TR, Haerty W, Higgs DR, Miska EA, Ponting CP. Bassett AR, et al. Elife. 2014 Aug 14;3:e03058. doi: 10.7554/eLife.03058. Elife. 2014. PMID: 25124674 Free PMC article. Review.
Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part r …
Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined …
Understanding functional miRNA-target interactions in vivo by site-specific genome engineering.
Bassett AR, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, Stanger N, Ewels PA, Taylor S, Ponting CP, Liu JL, Sauka-Spengler T, Fulga TA. Bassett AR, et al. Nat Commun. 2014 Aug 19;5:4640. doi: 10.1038/ncomms5640. Nat Commun. 2014. PMID: 25135198 Free PMC article.
MicroRNA (miRNA) target recognition is largely dictated by short 'seed' sequences, and single miRNAs therefore have the potential to regulate a large number of genes. ...
MicroRNA (miRNA) target recognition is largely dictated by short 'seed' sequences, and single miRNAs therefore have the potential to regulat …
A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.
Bassett AR, Kong L, Liu JL. Bassett AR, et al. J Genet Genomics. 2015 Jun 20;42(6):301-9. doi: 10.1016/j.jgg.2015.03.011. Epub 2015 Apr 18. J Genet Genomics. 2015. PMID: 26165496 Free PMC article.
In this study, we have designed and built a genome-wide CRISPR library covering 13,501 genes, among which 8989 genes are targeted by three or more independent single guide RNAs (sgRNAs). ...We hope that this library will provide an invaluable resource for the community to …
In this study, we have designed and built a genome-wide CRISPR library covering 13,501 genes, among which 8989 genes are targeted by …
CRISPR/Cas9 and genome editing in Drosophila.
Bassett AR, Liu JL. Bassett AR, et al. J Genet Genomics. 2014 Jan 20;41(1):7-19. doi: 10.1016/j.jgg.2013.12.004. Epub 2013 Dec 18. J Genet Genomics. 2014. PMID: 24480743 Review.
Recent advances in our ability to design DNA binding factors with specificity for desired sequences have resulted in a revolution in genetic engineering, enabling directed changes to the genome to be made relatively easily. ...
Recent advances in our ability to design DNA binding factors with specificity for desired sequences have resulted in a revolution in …
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.
Bassett AR, Tibbit C, Ponting CP, Liu JL. Bassett AR, et al. Biol Open. 2014 Jan 15;3(1):42-9. doi: 10.1242/bio.20137120. Biol Open. 2014. PMID: 24326186 Free PMC article.
By targeting a constitutive exon of the AGO1 gene, we demonstrate homozygous mutation in up to 82% of cells, thereby allowing the study of genetic knockouts in a Drosophila cell line for the first time. ...We demonstrate that a 1 kb homology arm le …
By targeting a constitutive exon of the AGO1 gene, we demonstrate homozygous mutation in up to 82% of cells, thereby allowing the stu …
Identification and properties of 1,119 candidate lincRNA loci in the Drosophila melanogaster genome.
Young RS, Marques AC, Tibbit C, Haerty W, Bassett AR, Liu JL, Ponting CP. Young RS, et al. Among authors: bassett ar. Genome Biol Evol. 2012;4(4):427-42. doi: 10.1093/gbe/evs020. Epub 2012 Mar 8. Genome Biol Evol. 2012. PMID: 22403033 Free PMC article.
Determining the biological relevance and potential gene regulatory mechanisms of these enigmatic molecules would be expedited in a more tractable model organism, such as Drosophila melanogaster. To this end, we defined a set of 1,119 putative lincRNA genes in D. mel …
Determining the biological relevance and potential gene regulatory mechanisms of these enigmatic molecules would be expedited in a mo …
Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.
Bassett AR, Tibbit C, Ponting CP, Liu JL. Bassett AR, et al. Cell Rep. 2013 Jul 11;4(1):220-8. doi: 10.1016/j.celrep.2013.06.020. Epub 2013 Jul 1. Cell Rep. 2013. PMID: 23827738 Free PMC article.
Our system provides at least a 10-fold improvement in efficiency over previously published reports, enabling wider application of this technique. We also describe a simple and highly sensitive method of detecting mutations in the target gene by high-resolution melt …
Our system provides at least a 10-fold improvement in efficiency over previously published reports, enabling wider application of thi …
Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes.
Bruntraeger M, Byrne M, Long K, Bassett AR. Bruntraeger M, et al. Among authors: bassett ar. Methods Mol Biol. 2019;1961:153-183. doi: 10.1007/978-1-4939-9170-9_11. Methods Mol Biol. 2019. PMID: 30912046
Its application to human induced pluripotent stem cells (hiPSCs) allows for the generation of isogenic cell pairs that differ in a single genetic lesion, and therefore the identification and characterization of causal genetic variants. ...We describe our use of a T7 …
Its application to human induced pluripotent stem cells (hiPSCs) allows for the generation of isogenic cell pairs that differ in a si …
454 results
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