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Phi 29 DNA polymerase active site. Mutants in conserved residues Tyr254 and Tyr390 are affected in dNTP binding.
Blasco MA, Lázaro JM, Bernad A, Blanco L, Salas M. Blasco MA, et al. Among authors: bernad a. J Biol Chem. 1992 Sep 25;267(27):19427-34. J Biol Chem. 1992. PMID: 1527062
Among them, the two conserved regions characterized by the amino acid motifs "D-NSLYP" and "K--NS(L/V)YG," regions 1 and 2a, respectively, according to Blanco et al. (Blanco, L., Bernad, A., Blasco, M. A. and Salas, M. (1991) Gene (Amst.) 100, 27-38) have bee …
Among them, the two conserved regions characterized by the amino acid motifs "D-NSLYP" and "K--NS(L/V)YG," regions 1 and 2a, respectively, a …
Evidence favouring the hypothesis of a conserved 3'-5' exonuclease active site in DNA-dependent DNA polymerases.
Blanco L, Bernad A, Salas M. Blanco L, et al. Among authors: bernad a. Gene. 1992 Mar 1;112(1):139-44. doi: 10.1016/0378-1119(92)90316-h. Gene. 1992. PMID: 1551594
The complete amino acid (aa) alignment of the N-terminal domain of 33 DNA-dependent DNA polymerases encompassing the putative segments Exo I, Exo II and Exo III, proposed by Bernad et al. [Cell 59 (1989) 219-228] to form a conserved 3'-5' exonuclease active site in …
The complete amino acid (aa) alignment of the N-terminal domain of 33 DNA-dependent DNA polymerases encompassing the putative segments Exo I …
A general structure for DNA-dependent DNA polymerases.
Blanco L, Bernad A, Blasco MA, Salas M. Blanco L, et al. Among authors: bernad a. Gene. 1991 Apr;100:27-38. doi: 10.1016/0378-1119(91)90346-d. Gene. 1991. PMID: 2055476
In addition to the general 3'-5' exonuclease domain described by Bernad et al. [Cell 59 (1989) 219-228] significant amino acid (aa) sequence similarity has been found in the C-terminal portion of 27 DNA-dependent DNA polymerases belonging to the two main superfamilies: (i) …
In addition to the general 3'-5' exonuclease domain described by Bernad et al. [Cell 59 (1989) 219-228] significant amino acid (aa) s …
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