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Role of replication in IS102-mediated deletion formation.
Bernardi F, Bernardi A. Bernardi F, et al. Mol Gen Genet. 1987 Oct;209(3):453-7. doi: 10.1007/BF00331149. Mol Gen Genet. 1987. PMID: 2828879
Intramolecular transposition produces replicon dissociation in a bireplicon; this reaction is homologous to the well-characterized IS-associated deletions in the case of a monoreplicon. However the frequencies at which these two reactions occur differ by a fa …
Intramolecular transposition produces replicon dissociation in a bireplicon; this reaction is homologous to the well-characterized IS …
Transcription of the target is required for IS102 mediated deletions.
Bernardi F, Bernardi A. Bernardi F, et al. Mol Gen Genet. 1988 May;212(2):265-70. doi: 10.1007/BF00334695. Mol Gen Genet. 1988. PMID: 2841569
We had previously shown that the cIII region of phage lambda is a target for IS102 mediated deletions and this region was therefore introduced in pSC101, where IS102 resides, in such a way that it was silent, transcribed or fully expressed. ...
We had previously shown that the cIII region of phage lambda is a target for IS102 mediated deletions and this region was therefore i …
Completed sequence of pKG1800, a vector for determination of transcription terminators.
Bernardi F, Bernardi A. Bernardi F, et al. DNA Seq. 1990;1(2):147-50. doi: 10.3109/10425179009016043. DNA Seq. 1990. PMID: 2134186
In this way the sequence of pKG1800, a vector constructed for detection of transcription terminator is now completed....
In this way the sequence of pKG1800, a vector constructed for detection of transcription terminator is now completed....
Inter- and intramolecular transposition of Tn903.
Bernardi F, Bernardi A. Bernardi F, et al. Mol Gen Genet. 1991 May;227(1):22-7. doi: 10.1007/BF00260701. Mol Gen Genet. 1991. PMID: 1646385
We have performed a detailed analysis of intra- and intermolecular endproducts of transposition of the compound transposon Tn903 and we show that, in our system, the transposition activity is almost entirely driven by one of the flanking insertion sequences, IS903L. ...IS9 …
We have performed a detailed analysis of intra- and intermolecular endproducts of transposition of the compound transposon Tn903 and …
Introduction of a UV-damaged replicon into a recipient cell is not a sufficient condition to produce an SOS-inducing signal.
Sommer S, Leitaõ A, Bernardi A, Bailone A, Devoret R. Sommer S, et al. Mutat Res. 1991 Mar;254(2):107-17. doi: 10.1016/0921-8777(91)90001-6. Mutat Res. 1991. PMID: 1825863
A UV-damaged oriC phagemid failed to induce SOS functions in a recipient cell whereas an oriF phagemid did induce them. Our results disprove the first and the second model proposed for the nature of the SOS-inducing signal. The failure of a UV-damaged oriC re
A UV-damaged oriC phagemid failed to induce SOS functions in a recipient cell whereas an oriF phagemid did induce them. Our re
Intramolecular transposition of IS102.
Bernardi F, Bernardi A. Bernardi F, et al. Gene. 1986;42(1):11-9. doi: 10.1016/0378-1119(86)90145-9. Gene. 1986. PMID: 3013726
However, the very low frequency (5 X 10(-8)) at which intramolecular transpositions in the bireplicons occurs, as compared to the single replicon (10(-4)), suggests that a complete transposition reaction may not be necessary to generate deletions....
However, the very low frequency (5 X 10(-8)) at which intramolecular transpositions in the bireplicons occurs, as compared to the single rep …
Atypical deletions generated by mutated IS102 elements.
Bernardi F, Bernardi A. Bernardi F, et al. Mol Gen Genet. 1984;195(3):452-8. doi: 10.1007/BF00341446. Mol Gen Genet. 1984. PMID: 6088945
We have introduced several mutations in the larger one near the COOH terminus and determined the residual ability of the mutated elements to generate deletions in order to assign a role to this polypeptide chain. We show that in these elements, deletions still occur, altho …
We have introduced several mutations in the larger one near the COOH terminus and determined the residual ability of the mutated elements to …
DNA rearrangements in a hybrid plasmid carrying the redB imm region of coliphage lambda.
Bernardi F, Bernardi A. Bernardi F, et al. Gene. 1980 Apr;9(1-2):13-25. doi: 10.1016/0378-1119(80)90164-x. Gene. 1980. PMID: 6247242
Restriction maps of these new plasmids indicate the following modifications: (1) the insertion of an IS1 element into gene N carried by the lambda fragment; (2) a mutation in the pL oL site of the same fragment, and (3) four large deletions (30 to 50% of the hybrid plasmid …
Restriction maps of these new plasmids indicate the following modifications: (1) the insertion of an IS1 element into gene N carried by the …
Site-specific deletions in the recombinant plasmid pSC101 containing the redB-ori region of phage lambda.
Bernardi A, Bernardi F. Bernardi A, et al. Gene. 1981 Jan-Feb;13(1):103-9. doi: 10.1016/0378-1119(81)90047-0. Gene. 1981. PMID: 6263749
Large deletions occur in the hybrid plasmid formed by pSC101 and the EcoRI fragment f2 of phage lambda (redB-ori region) under well defined growth conditions (Bernardi and Bernardi, 1980). ...
Large deletions occur in the hybrid plasmid formed by pSC101 and the EcoRI fragment f2 of phage lambda (redB-ori region) under well defined …
Properties of the catalytic domain of CDC25, a Saccharomyces cerevisiae GDP/GTP exchange factor: comparison of its activity on full-length and C-terminal truncated RAS2 proteins.
Jacquet E, Parrini MC, Bernardi A, Martegani E, Parmeggiani A. Jacquet E, et al. Biochem Biophys Res Commun. 1994 Mar 15;199(2):497-503. doi: 10.1006/bbrc.1994.1256. Biochem Biophys Res Commun. 1994. PMID: 8135791
RAS2-42/37kDa was somewhat more effective in enhancing the adenylylcyclase activity of a yeast membrane reconstituted system. CDC25-509 displayed a higher specific activity than the catalytic domains of the two CDC25-like proteins: S. cerevisiae SDC25 and mouse CDC2 …
RAS2-42/37kDa was somewhat more effective in enhancing the adenylylcyclase activity of a yeast membrane reconstituted system. CDC25-5 …
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