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Discovery of a steroid 11α-hydroxylase from Rhizopus oryzae and its biotechnological application.
Petrič S, Hakki T, Bernhardt R, Zigon D, Crešnar B. Petrič S, et al. Among authors: bernhardt r. J Biotechnol. 2010 Nov;150(3):428-37. doi: 10.1016/j.jbiotec.2010.09.928. Epub 2010 Sep 17. J Biotechnol. 2010. PMID: 20850485
To increase the 11α-hydroxylation activity of CYP509C12 in recombinant fission yeast, its natural redox partner, the R. oryzae NAD(P)H-dependent reductase, was coexpressed. ...
To increase the 11α-hydroxylation activity of CYP509C12 in recombinant fission yeast, its natural redox partner, the R. oryzae NAD(P) …
Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli.
Jose J, Bernhardt R, Hannemann F. Jose J, et al. Among authors: bernhardt r. J Biotechnol. 2002 May 23;95(3):257-68. doi: 10.1016/s0168-1656(02)00030-5. J Biotechnol. 2002. PMID: 12007866
Development of test systems for the discovery of selective human aldosterone synthase (CYP11B2) and 11beta-hydroxylase (CYP11B1) inhibitors. Discovery of a new lead compound for the therapy of congestive heart failure, myocardial fibrosis and hypertension.
Bureik M, Hübel K, Dragan CA, Scher J, Becker H, Lenz N, Bernhardt R. Bureik M, et al. Among authors: bernhardt r. Mol Cell Endocrinol. 2004 Mar 31;217(1-2):249-54. doi: 10.1016/j.mce.2003.10.027. Mol Cell Endocrinol. 2004. PMID: 15134825
The "Bringer" strategy: a very fast and highly efficient method for construction of mutant libraries by error-prone polymerase chain reaction of ring-closed plasmids.
Bichet A, Bureik M, Lenz N, Bernhardt R. Bichet A, et al. Among authors: bernhardt r. Appl Biochem Biotechnol. 2004 May;117(2):115-22. doi: 10.1385/abab:117:2:115. Appl Biochem Biotechnol. 2004. PMID: 15159555
Efficient conversion of 11-deoxycortisol to cortisol (hydrocortisone) by recombinant fission yeast Schizosaccharomyces pombe.
Drăgan CA, Zearo S, Hannemann F, Bernhardt R, Bureik M. Drăgan CA, et al. Among authors: bernhardt r. FEMS Yeast Res. 2005 Apr;5(6-7):621-5. doi: 10.1016/j.femsyr.2004.12.001. FEMS Yeast Res. 2005. PMID: 15780661
Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases.
Hannemann F, Virus C, Bernhardt R. Hannemann F, et al. Among authors: bernhardt r. J Biotechnol. 2006 Jun 25;124(1):172-81. doi: 10.1016/j.jbiotec.2006.01.009. Epub 2006 Feb 28. J Biotechnol. 2006. PMID: 16504331
Cytochromes P450 as versatile biocatalysts.
Bernhardt R. Bernhardt R. J Biotechnol. 2006 Jun 25;124(1):128-45. doi: 10.1016/j.jbiotec.2006.01.026. Epub 2006 Mar 3. J Biotechnol. 2006. PMID: 16516322 Review.
., Feyereisen, R., 2000. Cytochromes P450: a success story. Genome Biol. 1 (REVIEWS3003)), (Bernhardt, R., 2004. Cytochrome P-450. ...Chem. 1, 544-549), (Bernhardt, R., 2004. Optimized chimeragenesis; creating diverse P450 functions. Chem. Biol. …
., Feyereisen, R., 2000. Cytochromes P450: a success story. Genome Biol. 1 (REVIEWS3003)), (Bernhardt, R., 2004. Cytoch …
Coexpression of redox partners increases the hydrocortisone (cortisol) production efficiency in CYP11B1 expressing fission yeast Schizosaccharomyces pombe.
Hakki T, Zearo S, Drăgan CA, Bureik M, Bernhardt R. Hakki T, et al. Among authors: bernhardt r. J Biotechnol. 2008 Feb 1;133(3):351-9. doi: 10.1016/j.jbiotec.2007.06.022. Epub 2007 Jul 14. J Biotechnol. 2008. PMID: 17935813
Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli.
Zöllner A, Kagawa N, Waterman MR, Nonaka Y, Takio K, Shiro Y, Hannemann F, Bernhardt R. Zöllner A, et al. Among authors: bernhardt r. FEBS J. 2008 Feb;275(4):799-810. doi: 10.1111/j.1742-4658.2008.06253.x. Epub 2008 Jan 22. FEBS J. 2008. PMID: 18215163
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Girhard M, Klaus T, Khatri Y, Bernhardt R, Urlacher VB. Girhard M, et al. Among authors: bernhardt r. Appl Microbiol Biotechnol. 2010 Jun;87(2):595-607. doi: 10.1007/s00253-010-2472-z. Epub 2010 Feb 26. Appl Microbiol Biotechnol. 2010. PMID: 20186410
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