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Gating and conductance in an outward-rectifying K+ channel from the plasma membrane of Saccharomyces cerevisiae.
Bertl A, Slayman CL, Gradmann D. Bertl A, et al. J Membr Biol. 1993 Mar;132(3):183-99. doi: 10.1007/BF00235737. J Membr Biol. 1993. PMID: 8492306
The plasma membrane of the yeast Saccharomyces cerevisiae has been investigated by patch-clamp techniques, focusing upon the most conspicuous ion channel in that membrane, a K(+)-selective channel. ...Overall behavior of the channel is most simply described by a kin …
The plasma membrane of the yeast Saccharomyces cerevisiae has been investigated by patch-clamp techniques, focusing upon the most conspicuou …
Low-affinity potassium uptake by Saccharomyces cerevisiae is mediated by NSC1, a calcium-blocked non-specific cation channel.
Bihler H, Slayman CL, Bertl A. Bihler H, et al. Biochim Biophys Acta. 2002 Feb 1;1558(2):109-18. doi: 10.1016/s0005-2736(01)00414-x. Biochim Biophys Acta. 2002. PMID: 11779561
Slayman, A. Bertl, FEBS Lett. 432 (1998); S.K. Roberts, M. Fischer, G.K. Dixon, D.Sanders, J. Bacteriol. 181 (1999)) suggested that this inwardly rectifying pathway could relieve the growth inhibition normally imposed on yeast by disruption of its potassium transpor …
Slayman, A. Bertl, FEBS Lett. 432 (1998); S.K. Roberts, M. Fischer, G.K. Dixon, D.Sanders, J. Bacteriol. 181 (1999)) suggested …
From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
Gehwolf R, Weiss R, Gabler M, Hurst AC, Bertl A, Thalhamer J, Obermeyer G. Gehwolf R, et al. FEBS Lett. 2007 Feb 6;581(3):448-52. doi: 10.1016/j.febslet.2007.01.004. Epub 2007 Jan 16. FEBS Lett. 2007. PMID: 17239865 Free PMC article.
Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually ne …
Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were gen …
AtTPK4, an Arabidopsis tandem-pore K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+-dependent manner.
Becker D, Geiger D, Dunkel M, Roller A, Bertl A, Latz A, Carpaneto A, Dietrich P, Roelfsema MR, Voelker C, Schmidt D, Mueller-Roeber B, Czempinski K, Hedrich R. Becker D, et al. Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15621-6. doi: 10.1073/pnas.0401502101. Epub 2004 Oct 25. Proc Natl Acad Sci U S A. 2004. PMID: 15505206 Free PMC article.
AtTPK4 (KCO4) resembles the electrical properties of a voltage-independent K(+) channel after expression in Xenopus oocytes and yeast. ...The features of AtTPK4 point toward a role in potassium homeostasis and membrane voltage control of the growing pollen tube. ...
AtTPK4 (KCO4) resembles the electrical properties of a voltage-independent K(+) channel after expression in Xenopus oocytes and yeast …
The presumed potassium carrier Trk2p in Saccharomyces cerevisiae determines an H+-dependent, K+-independent current.
Bihler H, Gaber RF, Slayman CL, Bertl A. Bihler H, et al. FEBS Lett. 1999 Mar 19;447(1):115-20. doi: 10.1016/s0014-5793(99)00281-1. FEBS Lett. 1999. PMID: 10218594
That modulation could act either on the Trk2 protein itself or on interactions of Trk2 with other proteins in a hypothetical transporter complex. Structural considerations suggest a strong analogy to the KtrAB system in Vibrio alginolyticus and/or the TrkH system in …
That modulation could act either on the Trk2 protein itself or on interactions of Trk2 with other proteins in a hypothetical transpor …
NSC1: a novel high-current inward rectifier for cations in the plasma membrane of Saccharomyces cerevisiae.
Bihler H, Slayman CL, Bertl A. Bihler H, et al. FEBS Lett. 1998 Jul 31;432(1-2):59-64. doi: 10.1016/s0014-5793(98)00832-1. FEBS Lett. 1998. PMID: 9710251
The plasma membrane of the yeast Saccharomyces cerevisiae possesses a non-specific cation 'channel', tentatively dubbed NSC1, which is blocked by normal (mM) calcium and other divalent metal ions, is unblocked by reduction of extracellular free divalents below approximatel …
The plasma membrane of the yeast Saccharomyces cerevisiae possesses a non-specific cation 'channel', tentatively dubbed NSC1, which i …
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
Reid JD, Lukas W, Shafaatian R, Bertl A, Scheurmann-Kettner C, Guy HR, North RA. Reid JD, et al. Receptors Channels. 1996;4(1):51-62. Receptors Channels. 1996. PMID: 8723646
Potassium channel subunits have six or two transmembrane segments in addition to a conserved pore-forming (P) domain; four subunits come together to form a channel. A gene was identified in S. cerevisiae (J0911) encoding a protein with eight probable m …
Potassium channel subunits have six or two transmembrane segments in addition to a conserved pore-forming (P) domain; four subunits c …
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
Bertl A, Anderson JA, Slayman CL, Sentenac H, Gaber RF. Bertl A, et al. Folia Microbiol (Praha). 1994;39(6):507-9. doi: 10.1007/BF02814074. Folia Microbiol (Praha). 1994. PMID: 8550001
In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation o …
In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae …
Use of Saccharomyces cerevisiae for patch-clamp analysis of heterologous membrane proteins: characterization of Kat1, an inward-rectifying K+ channel from Arabidopsis thaliana, and comparison with endogeneous yeast channels and carriers.
Bertl A, Anderson JA, Slayman CL, Gaber RF. Bertl A, et al. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2701-5. doi: 10.1073/pnas.92.7.2701. Proc Natl Acad Sci U S A. 1995. PMID: 7708709 Free PMC article.
The present study uses whole-cell patch-clamp experiments to show that yeast strains which grow poorly on submillimolar K+ due to the deletion of two K(+)-transporter genes (TRK1 and TRK2) are in fact missing a prominent K+ inward current present in wild-type cells. Rescue …
The present study uses whole-cell patch-clamp experiments to show that yeast strains which grow poorly on submillimolar K+ due to the deleti …
Modes and means of channel behavior: an introduction.
Slayman CL, Bertl A. Slayman CL, et al. Symp Soc Exp Biol. 1994;48:77-83. Symp Soc Exp Biol. 1994. PMID: 7541164 Review.
A flush of technical developments and the resultant burgeoning research on channel molecules in biological membranes has, over the past two decades, led to clear operational distinctions between channel behavior and carrier behavior. ...Although a full survey of the
A flush of technical developments and the resultant burgeoning research on channel molecules in biological membranes has, over the pa
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