Toxicopathological evaluation in Wistar rats (Rattus norvegicus) following repeated oral exposure to acephate

Amit R Bhadaniya; Vinay A Kalariya; Dilip V Joshi; Bakor J Patel; Sandhya Chaudhary; Hitesh B Patel; Jignesh M Patel; Urvesh D Patel; Harshad B Patel; Sanjay N Ghodasara; Harish H Savsani

doi:10.1177/0748233712468017

Toxicopathological evaluation in Wistar rats (Rattus norvegicus) following repeated oral exposure to acephate

Toxicol Ind Health. 2015 Jan;31(1):9-17. doi: 10.1177/0748233712468017. Epub 2012 Dec 4.

Authors

Affiliations

¹ Department of Veterinary Pathology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India arbhadaniya@yahoo.co.in.
² Department of Veterinary Pathology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India.
³ Department of Veterinary Pathology, College of Veterinary Science & A.H., Sardarkrushinagar Dantiwada Agricultural University, Dantiwada, Gujarat, India.
⁴ Department of Veterinary Physiology, College of Veterinary Science & A.H., Sardarkrushinagar Dantiwada Agricultural University, Dantiwada, Gujarat, India.
⁵ Department of Pharmacology and Toxicology, College of Veterinary Science & A.H., Sardarkrushinagar Dantiwada Agricultural University, Dantiwada, Gujarat, India.
⁶ Department of Veterinary Pathology, College of Veterinary Science & A.H., Navsari Agricultural University, Navsari, Gujarat, India.
⁷ Department of Pharmacology and Toxicology, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India.
⁸ Cattle Breeding Farm, Junagadh Agricultural University, Junagadh, Gujarat, India.
⁹ Department of Animal Nutrition, College of Veterinary Science & A.H., Junagadh Agricultural University, Junagadh, Gujarat, India.

PMID: 23211677
DOI: 10.1177/0748233712468017

Abstract

The present study was carried out to evaluate the effects of exposure at different doses of acephate on hematology, blood biochemistry, oxidative stress and immune system of Wistar rats. The experiment was carried out on 40 Wistar rats, which were divided in four groups. Animals of the three treatment groups were given with different sublethal doses (1/40th, 1/20th, 1/10th of lethal dose 50 value) of acephate by oral gavage. The hematology, blood biochemistry, oxidative stress marker, humoral immune response and cell-mediated immunity were evaluated following acephate exposure. Significant alteration in hematological parameters was not observed following different doses of acephate; however, significant alteration in alkaline phosphatase, gamma glutamyl transferase, acetyl cholinesterase, lipid peroxidase and superoxide dismutase was observed in medium- and high-dose group animals. Nonsignificant decrease in antibody titer in animals exposed to high dose has been observed compared with animals of control group. However, significant alteration in cell-mediated immunity was not observed in animals treated with acephate at different doses.

Keywords: Toxicity; acephate; immunity; pathology; rats; repeated exposure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Blood Chemical Analysis
Female
Immunity / drug effects
Male
Organothiophosphorus Compounds / administration & dosage
Organothiophosphorus Compounds / toxicity*
Phosphoramides / administration & dosage
Phosphoramides / toxicity*
Rats
Rats, Wistar
Toxicity Tests, Subchronic

Substances

Organothiophosphorus Compounds
Phosphoramides
acephate