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Analytical performances of SPAPLUS® turbidimeter for the quantification of albumin and IgG in serum and cerebrospinal fluid.
Benard C, Chauzeix J, Blancher A, Puissant-Lubrano B. Benard C, et al. Among authors: blancher a. Clin Biochem. 2016 Jul;49(10-11):828-31. doi: 10.1016/j.clinbiochem.2016.03.008. Epub 2016 Apr 9. Clin Biochem. 2016. PMID: 27048976
The confrontation of CSF IgG index and isoelectric focusing results led to a sensitivity of 79% and a specificity of 97% of CSF IgG index quantified on SPAPLUS® for the presence of oligoclonal bands at IEF. ...
The confrontation of CSF IgG index and isoelectric focusing results led to a sensitivity of 79% and a specificity of 97% of CS …
A microplate assay to measure classical and alternative complement activity.
Puissant-Lubrano B, Fortenfant F, Winterton P, Blancher A. Puissant-Lubrano B, et al. Among authors: blancher a. Clin Chem Lab Med. 2017 May 1;55(6):845-853. doi: 10.1515/cclm-2016-0553. Clin Chem Lab Med. 2017. PMID: 28125402
BACKGROUND: We developed and validated a kinetic microplate hemolytic assay (HA) to quantify classical and alternative complement activity in a single dilution of human plasma or serum. ...CONCLUSIONS: This assay allows the simultaneous measurement of 36 samples in …
BACKGROUND: We developed and validated a kinetic microplate hemolytic assay (HA) to quantify classical and alternative complement act …
[Analytical performances of SPAPLUS® turbidimeter for the dosage of immunoglobulins and beta-2 microglobulin in serum].
Thevenet I, Benat C, Chauzeix J, Blancher A, Puissant-Lubrano B. Thevenet I, et al. Among authors: blancher a. Ann Biol Clin (Paris). 2014 Jul-Aug;72(4):423-34. doi: 10.1684/abc.2014.0974. Ann Biol Clin (Paris). 2014. PMID: 25119800 French.
The within-day precision (repeatability) and the between-day precision (reproducibility) were obtained for two or three concentration levels depending of the parameter and were below the recommendations of the manufacturer, except for the repeatability of IgG1 (at a level …
The within-day precision (repeatability) and the between-day precision (reproducibility) were obtained for two or three concentration levels …
Prevalence of cryoglobulinemia and autoimmunity markers in renal-transplant patients.
Faguer S, Kamar N, Boulestin A, Esposito L, Durand D, Blancher A, Rostaing L. Faguer S, et al. Among authors: blancher a. Clin Nephrol. 2008 Apr;69(4):239-43. doi: 10.5414/cnp69239. Clin Nephrol. 2008. PMID: 18397697
AIMS: To examine the prevalence of cryoglobulinemia (Cryo) and autoimmune markers in renal-transplant recipients in a stable condition, and to determine its risk factors and impact upon allograft function. ...Cryo(+) RT patients had been recipients of a graft for lo …
AIMS: To examine the prevalence of cryoglobulinemia (Cryo) and autoimmune markers in renal-transplant recipients in a stable conditio …
Autoantibodies in serum and CSF of patients with multiple sclerosis.
Blancher A, Matsiota P, Guilbert B, Doyon B, Clanet M, Kouvelas E, Rascol A, Avrameas S. Blancher A, et al. Ann N Y Acad Sci. 1988;540:290-2. doi: 10.1111/j.1749-6632.1988.tb27078.x. Ann N Y Acad Sci. 1988. PMID: 3207257 No abstract available.
Polymerase chain reaction on cerebrospinal fluid cells in the detection of leptomeningeal involvement by B-cell lymphoma and leukaemia: a novel strategy and its implications.
Galoin S, Daste G, Apoil PA, Chollet F, Roda D, Blancher A, Delsol G, Chittal S, al Saati T. Galoin S, et al. Among authors: blancher a. Br J Haematol. 1997 Oct;99(1):122-30. doi: 10.1046/j.1365-2141.1997.3423153.x. Br J Haematol. 1997. PMID: 9359512
This method detects a rearranged IgH gene from a single B lymphocyte which may or may not represent the neoplastic B-cell population. We therefore performed multiple snPCR (three to five) experiments on the same CSF sample, postulating that the detection of a
This method detects a rearranged IgH gene from a single B lymphocyte which may or may not represent the neoplastic B-cell popu …
Alternative complement pathway hemolytic assays reveal incomplete complement blockade in patients treated with eculizumab.
Puissant-Lubrano B, Puissochet S, Congy-Jolivet N, Chauveau D, Decramer S, Garnier A, Huart A, Kamar N, Ribes D, Blancher A. Puissant-Lubrano B, et al. Among authors: blancher a. Clin Immunol. 2017 Oct;183:1-7. doi: 10.1016/j.clim.2017.06.007. Epub 2017 Jun 21. Clin Immunol. 2017. PMID: 28647502
Conversely, functional ELISA revealed a complete blockade of TP through AP activation in all patients (<10%). C5a and sC5b9 levels were not correlated with residual APH50 or AP100. Similar results were obtained after in vitro addition of increasing amounts of eculizumab …
Conversely, functional ELISA revealed a complete blockade of TP through AP activation in all patients (<10%). C5a and sC5b9 levels …
Comparative study of natural autoantibodies in the serum and cerebrospinal fluid of normal individuals and patients with multiple sclerosis and other neurological diseases.
Matsiota P, Blancher A, Doyon B, Guilbert B, Clanet M, Kouvelas ED, Avrameas S. Matsiota P, et al. Among authors: blancher a. Ann Inst Pasteur Immunol. 1988 Jan-Feb;139(1):99-108. doi: 10.1016/0769-2625(88)90134-1. Ann Inst Pasteur Immunol. 1988. PMID: 3258758
Restricted antibody activity against a given antigen of the panel was not observed. Compared to the two other groups, the MS group had equivalent titres of anti-MBP antibodies in the CSF. These results suggest that, in MS, a general immune dysregulation exists which …
Restricted antibody activity against a given antigen of the panel was not observed. Compared to the two other groups, the MS group ha …
Human monoclonal autoantibodies produced by hybridomas derived from lymphocytes of multiple sclerosis patients.
Blancher A, Oksman F, Lymberi P, Calvas P, Cambon-Thomsen A, Clanet M, Ducos J. Blancher A, et al. Res Immunol. 1989 Sep;140(7):711-24. doi: 10.1016/0923-2494(89)90024-2. Res Immunol. 1989. PMID: 2595082
Anti-tissue reactivity was assessed by indirect immunofluorescence assay (IFA) on human cerebellum and peripheral nerve as well as on a panel of 8 non-nervous tissues. ...Moreover, cross-reactivity was observed with a hapten (TNP-BSA)....
Anti-tissue reactivity was assessed by indirect immunofluorescence assay (IFA) on human cerebellum and peripheral nerve as well as on a
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