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Structural and functional studies on phi 29 DNA polymerase.
Blasco MA, Esteban JA, Méndez J, Blanco L, Salas M. Blasco MA, et al. Among authors: blanco l. Chromosoma. 1992;102(1 Suppl):S32-8. doi: 10.1007/BF02451783. Chromosoma. 1992. PMID: 1291240
In vitro replication of bacteriophage PRD1 DNA. Metal activation of protein-primed initiation and DNA elongation.
Caldentey J, Blanco L, Savilahti H, Bamford DH, Salas M. Caldentey J, et al. Among authors: blanco l. Nucleic Acids Res. 1992 Aug 11;20(15):3971-6. doi: 10.1093/nar/20.15.3971. Nucleic Acids Res. 1992. PMID: 1324473 Free PMC article.
Site-directed mutagenesis at the Exo III motif of phi 29 DNA polymerase; overlapping structural domains for the 3'-5' exonuclease and strand-displacement activities.
Soengas MS, Esteban JA, Lázaro JM, Bernad A, Blasco MA, Salas M, Blanco L. Soengas MS, et al. Among authors: blanco l. EMBO J. 1992 Nov;11(11):4227-37. EMBO J. 1992. PMID: 1396603 Free PMC article.
Activation of S1 nuclease at neutral pH.
Esteban JA, Salas M, Blanco L. Esteban JA, et al. Among authors: blanco l. Nucleic Acids Res. 1992 Sep 25;20(18):4932. doi: 10.1093/nar/20.18.4932. Nucleic Acids Res. 1992. PMID: 1408810 Free PMC article.
Initiation of phi 29 DNA replication occurs at the second 3' nucleotide of the linear template: a sliding-back mechanism for protein-primed DNA replication.
Méndez J, Blanco L, Esteban JA, Bernad A, Salas M. Méndez J, et al. Among authors: blanco l. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9579-83. doi: 10.1073/pnas.89.20.9579. Proc Natl Acad Sci U S A. 1992. PMID: 1409668 Free PMC article.
Phi 29 DNA polymerase active site. Mutants in conserved residues Tyr254 and Tyr390 are affected in dNTP binding.
Blasco MA, Lázaro JM, Bernad A, Blanco L, Salas M. Blasco MA, et al. Among authors: blanco l. J Biol Chem. 1992 Sep 25;267(27):19427-34. J Biol Chem. 1992. PMID: 1527062
Among them, the two conserved regions characterized by the amino acid motifs "D-NSLYP" and "K--NS(L/V)YG," regions 1 and 2a, respectively, according to Blanco et al. (Blanco, L., Bernad, A., Blasco, M. A. and Salas, M. (1991) Gene (Amst.) 100, 27-38) h …
Among them, the two conserved regions characterized by the amino acid motifs "D-NSLYP" and "K--NS(L/V)YG," regions 1 and 2a, respecti …
DNA-independent deoxynucleotidylation of the phi 29 terminal protein by the phi 29 DNA polymerase.
Blanco L, Bernad A, Esteban JA, Salas M. Blanco L, et al. J Biol Chem. 1992 Jan 15;267(2):1225-30. J Biol Chem. 1992. PMID: 1730646
The nature of the TP-dAMP linkage was shown to be identical (a O-5'-deoxyadenylyl-L-serine bond) to that found covalently linking TP to the DNA of bacteriophage phi 29, indicating that this DNA-independent reaction actually mimics that occurring as the initiation step of p …
The nature of the TP-dAMP linkage was shown to be identical (a O-5'-deoxyadenylyl-L-serine bond) to that found covalently linking TP …
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