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High-level production of tetraacetyl phytosphingosine (TAPS) by combined genetic engineering of sphingoid base biosynthesis and L-serine availability in the non-conventional yeast Pichia ciferrii.
Schorsch C, Köhler T, Andrea H, Boles E. Schorsch C, et al. Metab Eng. 2012 Mar;14(2):172-84. doi: 10.1016/j.ymben.2011.12.002. Epub 2011 Dec 10. Metab Eng. 2012. PMID: 22178746
Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae.
Brat D, Boles E, Wiedemann B. Brat D, et al. Appl Environ Microbiol. 2009 Apr;75(8):2304-11. doi: 10.1128/AEM.02522-08. Epub 2009 Feb 13. Appl Environ Microbiol. 2009. PMID: 19218403 Free PMC article.
Metabolic engineering of the non-conventional yeast Pichia ciferrii for production of rare sphingoid bases.
Börgel D, van den Berg M, Hüller T, Andrea H, Liebisch G, Boles E, Schorsch C, van der Pol R, Arink A, Boogers I, van der Hoeven R, Korevaar K, Farwick M, Köhler T, Schaffer S. Börgel D, et al. Metab Eng. 2012 Jul;14(4):412-26. doi: 10.1016/j.ymben.2012.03.003. Epub 2012 Mar 18. Metab Eng. 2012. PMID: 22449569
In order to convert the phytosphingosine-producing P. ciferrii wildtype into a strain capable of producing predominantly sphinganine, Syringomycin E-resistant mutants were isolated. ...
In order to convert the phytosphingosine-producing P. ciferrii wildtype into a strain capable of producing predominantly sphinganine, Syring …
Cytosolic re-localization and optimization of valine synthesis and catabolism enables inseased isobutanol production with the yeast Saccharomyces cerevisiae.
Brat D, Weber C, Lorenzen W, Bode HB, Boles E. Brat D, et al. Biotechnol Biofuels. 2012 Sep 6;5(1):65. doi: 10.1186/1754-6834-5-65. Biotechnol Biofuels. 2012. PMID: 22954227 Free PMC article.
Biosynthesis of cis,cis-muconic acid and its aromatic precursors, catechol and protocatechuic acid, from renewable feedstocks by Saccharomyces cerevisiae.
Weber C, Brückner C, Weinreb S, Lehr C, Essl C, Boles E. Weber C, et al. Appl Environ Microbiol. 2012 Dec;78(23):8421-30. doi: 10.1128/AEM.01983-12. Epub 2012 Sep 21. Appl Environ Microbiol. 2012. PMID: 23001678 Free PMC article.
Isobutanol production from D-xylose by recombinant Saccharomyces cerevisiae.
Brat D, Boles E. Brat D, et al. FEMS Yeast Res. 2013 Mar;13(2):241-4. doi: 10.1111/1567-1364.12028. Epub 2013 Jan 16. FEMS Yeast Res. 2013. PMID: 23279585
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