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Rapid colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens.
Lage AP, Fauconnier A, Burette A, Glupczynski Y, Bollen A, Godfroid E. Lage AP, et al. J Clin Microbiol. 1996 Mar;34(3):530-3. doi: 10.1128/JCM.34.3.530-533.1996. J Clin Microbiol. 1996. PMID: 8904408 Free PMC article.
A very simple, practical, sensitive, and specific colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA is described. This assay, which combines a sensitive sandwich DNA hybridization reaction and a colorimetric protocol similar to
A very simple, practical, sensitive, and specific colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA is
Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens.
Lage AP, Godfroid E, Fauconnier A, Burette A, Butzler JP, Bollen A, Glupczynski Y. Lage AP, et al. J Clin Microbiol. 1995 Oct;33(10):2752-6. doi: 10.1128/JCM.33.10.2752-2756.1995. J Clin Microbiol. 1995. PMID: 8567918 Free PMC article.
A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease test, and Giemsa staining of hist
A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (h
Characterization of the type III secretion locus of Bordetella pertussis.
Fauconnier A, Veithen A, Gueirard P, Antoine R, Wacheul L, Locht C, Bollen A, Godfroid E. Fauconnier A, et al. Int J Med Microbiol. 2001 Mar;290(8):693-705. doi: 10.1016/S1438-4221(01)80009-6. Int J Med Microbiol. 2001. PMID: 11310448
Amplification of Bordetella pertussis DNA with these primers yielded a fragment that was further used as a probe for screening a genomic library. The nucleotide sequence of a positive clone revealed a 2100-bp gene, called bcrD, which specifies …
Amplification of Bordetella pertussis DNA with these primers yielded a fragment that was further used as a probe for screening …
Specific identification of Bordetella pertussis by the polymerase chain reaction.
Houard S, Hackel C, Herzog A, Bollen A. Houard S, et al. Res Microbiol. 1989 Sep;140(7):477-87. doi: 10.1016/0923-2508(89)90069-7. Res Microbiol. 1989. PMID: 2560238
One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. …
One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a
Specific identification of Mycobacterium leprae by the polymerase chain reaction.
Hackel C, Houard S, Portaels F, van Elsen A, Herzog A, Bollen A. Hackel C, et al. Mol Cell Probes. 1990 Jun;4(3):205-10. doi: 10.1016/0890-8508(90)90054-4. Mol Cell Probes. 1990. PMID: 2199822
A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa antigen of M. leprae. A second pair of primers, targetted to the same gene, leads to the amplification of a 154 bp DNA piece cons
A first set of primers, PLp1 and PLp2, identifies a specific 386 bp DNA fragment located in the gene coding for the 65 kDa ant
Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay.
Debue M, Gautier P, Hackel C, Van Elsen A, Herzog A, Bigaignon G, Bollen A. Debue M, et al. Res Microbiol. 1991 Jun;142(5):565-72. doi: 10.1016/0923-2508(91)90189-h. Res Microbiol. 1991. PMID: 1947428
One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. ...
One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece …
Rapid identification of Mycobacterium xenopi from bacterial colonies or "Bactec" culture by the polymerase chain reaction and a luminescent sandwich hybridization assay.
Fauville-Dufaux M, Maes N, Severin E, Farin C, Serruys E, Struelens M, Younes N, Vincke JP, De Vos MJ, Bollen A, et al. Fauville-Dufaux M, et al. Res Microbiol. 1995 May;146(4):349-56. doi: 10.1016/0923-2508(96)81058-8. Res Microbiol. 1995. PMID: 7569329
Oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a specific 584-bp DNA fragment, located in the 16S RNA gene of Mycobacterium xenopi. ...In addition, a luminescent hybridization assay was designed for use on PCR-amplified DNA. This …
Oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a specific 584-bp DNA fragment, located in the 16 …
PCR-reverse line blot typing method underscores the genomic heterogeneity of Borrelia valaisiana species and suggests its potential involvement in Lyme disease.
Godfroid E, Min Hu C, Humair PF, Bollen A, Gern L. Godfroid E, et al. J Clin Microbiol. 2003 Aug;41(8):3690-8. doi: 10.1128/jcm.41.8.3690-3698.2003. J Clin Microbiol. 2003. PMID: 12904377 Free PMC article.
From a phylogenetic analysis on a great number of ospA gene sequences, we have designed and synthesized a set of PCR primers specific to the five Borrelia burgdorferi sensu lato genospecies present in Europe and a subset of probes (capture and detectio …
From a phylogenetic analysis on a great number of ospA gene sequences, we have designed and synthesized a set of PCR pr …
Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients.
Demaerschalck I, Ben Messaoud A, De Kesel M, Hoyois B, Lobet Y, Hoet P, Bigaignon G, Bollen A, Godfroid E. Demaerschalck I, et al. J Clin Microbiol. 1995 Mar;33(3):602-8. doi: 10.1128/JCM.33.3.602-608.1995. J Clin Microbiol. 1995. PMID: 7538507 Free PMC article.
In toto, 10 patients appeared to have been infected by a single genospecies and 8 were infected by more than one Lyme disease-associated genospecies. ...
In toto, 10 patients appeared to have been infected by a single genospecies and 8 were infected by more than one Lyme disease-associa …
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