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Page 1
Trauma-related Lyme arthritis.
Roberts J 4th, Merchant E. Roberts J 4th, et al. BMJ Case Rep. 2023 Jul 5;16(7):e255532. doi: 10.1136/bcr-2023-255532. BMJ Case Rep. 2023. PMID: 37407232 Free PMC article.
Aspiration of the left knee joint revealed a white cell count of 21.0 10(9)/L (83% neutrophils) with negative Gram stain and culture. However, Lyme PCR was positive and accompanied by serologies consistent with Lyme arthritis. He was treated with a standard course of anti …
Aspiration of the left knee joint revealed a white cell count of 21.0 10(9)/L (83% neutrophils) with negative Gram stain and culture. Howev …
Detection of Borrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis.
Nocton JJ, Dressler F, Rutledge BJ, Rys PN, Persing DH, Steere AC. Nocton JJ, et al. N Engl J Med. 1994 Jan 27;330(4):229-34. doi: 10.1056/NEJM199401273300401. N Engl J Med. 1994. PMID: 8272083 Free article.
BACKGROUND: Borrelia burgdorferi is difficult to detect in synovial fluid, which limits our understanding of the pathogenesis of Lyme arthritis, particularly when arthritis persists despite antibiotic therapy. METHODS: Using the polymerase chain reacti …
BACKGROUND: Borrelia burgdorferi is difficult to detect in synovial fluid, which limits our understanding of the …
Isolation and detection of Borrelia burgdorferi DNA from cerebral spinal fluid, synovial fluid, blood, urine, and ticks using the Roche MagNA Pure system and real-time PCR.
Exner MM, Lewinski MA. Exner MM, et al. Diagn Microbiol Infect Dis. 2003 Aug;46(4):235-40. doi: 10.1016/s0732-8893(03)00080-4. Diagn Microbiol Infect Dis. 2003. PMID: 12944012
The Roche MagNA Pure automated nucleic acid extraction system was tested for its ability to extract Borrelia burgdorferi DNA from a diverse set of spiked specimen types including blood, cerebral spinal fluid, synovial fluid, urine and ticks. ... …
The Roche MagNA Pure automated nucleic acid extraction system was tested for its ability to extract Borrelia burgdorferi DNA f …
Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay.
Schwaiger M, Péter O, Cassinotti P. Schwaiger M, et al. Clin Microbiol Infect. 2001 Sep;7(9):461-9. doi: 10.1046/j.1198-743x.2001.00282.x. Clin Microbiol Infect. 2001. PMID: 11678928 Free article.
OBJECTIVE: To establish a one-tube fluorogenic real-time PCR assay for routine detection of Borrelia burgdorferi (sensu lato) DNA in various clinical specimens. ...Borrelia burgdorferi DNA was also detected in five (17.9%) of 28 synovial …
OBJECTIVE: To establish a one-tube fluorogenic real-time PCR assay for routine detection of Borrelia burgdorferi (sensu …
Detection of Borrelia burgdorferi by polymerase chain reaction in synovial membrane, but not in synovial fluid from patients with persisting Lyme arthritis after antibiotic therapy.
Priem S, Burmester GR, Kamradt T, Wolbart K, Rittig MG, Krause A. Priem S, et al. Ann Rheum Dis. 1998 Feb;57(2):118-21. doi: 10.1136/ard.57.2.118. Ann Rheum Dis. 1998. PMID: 9613343 Free PMC article.
OBJECTIVES: To identify possible sites of bacterial persistence in patients with treatment resistant Lyme arthritis. It was determined whether Borrelia burgdorferi DNA may be detectable by polymerase chain reaction (PCR) in synovial membrane (SM) when …
OBJECTIVES: To identify possible sites of bacterial persistence in patients with treatment resistant Lyme arthritis. It was determined wheth …
Heterogeneity of Borrelia burgdorferi sensu lato demonstrated by an ospA-type-specific PCR in synovial fluid from patients with Lyme arthritis.
Vasiliu V, Herzer P, Rössler D, Lehnert G, Wilske B. Vasiliu V, et al. Med Microbiol Immunol. 1998 Oct;187(2):97-102. doi: 10.1007/s004300050079. Med Microbiol Immunol. 1998. PMID: 9832323
Borrelia burgdorferi sensu lato, the etiological agent of Lyme borreliosis, has been divided into three genospecies: B. burgdorferi sensu stricto (OspA-type 1), B. afzelii (OspA-type 2) and B. garinii (OspA-type 3-7). ...Our study group consisted of 20 patien …
Borrelia burgdorferi sensu lato, the etiological agent of Lyme borreliosis, has been divided into three genospecies: B. bur …
Differentiating Lyme arthritis: a case-based review.
Unlu AM, Andersen NS, Larsen SL, Skarphedinsson S, Chrysidis S, Knudtzen FC, Lage-Hansen PR. Unlu AM, et al. Rheumatol Int. 2024 Nov;44(11):2671-2678. doi: 10.1007/s00296-024-05618-0. Epub 2024 May 25. Rheumatol Int. 2024. PMID: 38795123
Our case series identified six patients with LA from a localized region in Southern Denmark; all confirmed by Borrelia-specific real-time PCR from synovial fluid. The diagnostic delay was up to 38 months. ...Lack of tick exposure history, antibody asse …
Our case series identified six patients with LA from a localized region in Southern Denmark; all confirmed by Borrelia-specific real- …
Diagnosis of lyme borreliosis.
Aguero-Rosenfeld ME, Wang G, Schwartz I, Wormser GP. Aguero-Rosenfeld ME, et al. Clin Microbiol Rev. 2005 Jul;18(3):484-509. doi: 10.1128/CMR.18.3.484-509.2005. Clin Microbiol Rev. 2005. PMID: 16020686 Free PMC article. Review.
PCR on material obtained from extracutaneous sites is in general of low sensitivity, with the exception of synovial fluid. PCR on synovial fluid has shown a sensitivity of up to >90% (when using four different primer sets) in patients …
PCR on material obtained from extracutaneous sites is in general of low sensitivity, with the exception of synovial fluid …
Chlamydia and Borrelia DNA in synovial fluid of patients with early undifferentiated oligoarthritis: results of a prospective study.
Schnarr S, Putschky N, Jendro MC, Zeidler H, Hammer M, Kuipers JG, Wollenhaupt J. Schnarr S, et al. Arthritis Rheum. 2001 Nov;44(11):2679-85. doi: 10.1002/1529-0131(200111)44:11<2679::aid-art447>3.0.co;2-c. Arthritis Rheum. 2001. PMID: 11710723
Patients were examined by standardized clinical and immunoserologic procedures. Synovial fluid was screened for the presence of Ct and Bb DNA by polymerase chain reaction (PCR). ...The frequency of bacteria-specific DNA was 50% (7 of 14) in CIA patients and 6 …
Patients were examined by standardized clinical and immunoserologic procedures. Synovial fluid was screened for the presence o …
Direct molecular typing of Borrelia burgdorferi sensu lato species in synovial samples from patients with lyme arthritis.
Jaulhac B, Heller R, Limbach FX, Hansmann Y, Lipsker D, Monteil H, Sibilia J, Piémont Y. Jaulhac B, et al. J Clin Microbiol. 2000 May;38(5):1895-900. doi: 10.1128/JCM.38.5.1895-1900.2000. J Clin Microbiol. 2000. PMID: 10790118 Free PMC article.
However, very few strains of the causative bacterium, Borrelia burgdorferi, have been isolated from synovial samples. For this reason, different molecular direct typing methods were developed recently to assess which species could be involved in Lyme arthriti …
However, very few strains of the causative bacterium, Borrelia burgdorferi, have been isolated from synovial samples. F …
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