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Analysis of S100 oligomers and amyloids.
Botelho HM, Fritz G, Gomes CM. Botelho HM, et al. Methods Mol Biol. 2012;849:373-86. doi: 10.1007/978-1-61779-551-0_25. Methods Mol Biol. 2012. PMID: 22528103
Intrinsically disordered and aggregation prone regions underlie β-aggregation in S100 proteins.
Carvalho SB, Botelho HM, Leal SS, Cardoso I, Fritz G, Gomes CM. Carvalho SB, et al. Among authors: botelho hm. PLoS One. 2013 Oct 1;8(10):e76629. doi: 10.1371/journal.pone.0076629. eCollection 2013. PLoS One. 2013. PMID: 24098542 Free PMC article.
The neuronal S100B protein is a calcium-tuned suppressor of amyloid-β aggregation.
Cristóvão JS, Morris VK, Cardoso I, Leal SS, Martínez J, Botelho HM, Göbl C, David R, Kierdorf K, Alemi M, Madl T, Fritz G, Reif B, Gomes CM. Cristóvão JS, et al. Among authors: botelho hm. Sci Adv. 2018 Jun 29;4(6):eaaq1702. doi: 10.1126/sciadv.aaq1702. eCollection 2018 Jun. Sci Adv. 2018. PMID: 29963623 Free PMC article.
S100A6 amyloid fibril formation is calcium-modulated and enhances superoxide dismutase-1 (SOD1) aggregation.
Botelho HM, Leal SS, Cardoso I, Yanamandra K, Morozova-Roche LA, Fritz G, Gomes CM. Botelho HM, et al. J Biol Chem. 2012 Dec 7;287(50):42233-42. doi: 10.1074/jbc.M112.396416. Epub 2012 Oct 17. J Biol Chem. 2012. PMID: 23076148 Free PMC article.
Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).
Amaral MD, Farinha CM, Matos P, Botelho HM. Amaral MD, et al. Among authors: botelho hm. Methods Mol Biol. 2016;1459:105-26. doi: 10.1007/978-1-4939-3804-9_7. Methods Mol Biol. 2016. PMID: 27665554
A proteomic approach toward the selection of proteins with enhanced intrinsic conformational stability.
Prosinecki V, Botelho HM, Francese S, Mastrobuoni G, Moneti G, Urich T, Kletzin A, Gomes CM. Prosinecki V, et al. Among authors: botelho hm. J Proteome Res. 2006 Oct;5(10):2720-6. doi: 10.1021/pr0602491. J Proteome Res. 2006. PMID: 17022643
Structural reorganization renders enhanced metalloprotein stability.
Botelho HM, Gomes CM. Botelho HM, et al. Chem Commun (Camb). 2011 Oct 21;47(39):11149-51. doi: 10.1039/c1cc13354c. Epub 2011 Sep 6. Chem Commun (Camb). 2011. PMID: 21894348
Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies.
Palma E, Botelho HM, Morais GR, Rodrigues I, Santos IC, Campello MPC, Raposinho P, Belchior A, Gomes SS, Araújo MF, Correia I, Ribeiro N, Gama S, Mendes F, Paulo A. Palma E, et al. Among authors: botelho hm. J Biol Inorg Chem. 2019 Feb;24(1):71-89. doi: 10.1007/s00775-018-1629-6. Epub 2018 Nov 24. J Biol Inorg Chem. 2019. PMID: 30474755
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