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An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples.
Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Brown Jordan A, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, Dionísio TJ, Garbieri TF, Parisi VA, Mendes-Correa MC, de Paula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. Mills MG, et al. Among authors: botten j. PLoS One. 2022 Jan 13;17(1):e0261853. doi: 10.1371/journal.pone.0261853. eCollection 2022. PLoS One. 2022. PMID: 35025926 Free PMC article.
A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR).
King BR, Hershkowitz D, Eisenhauer PL, Weir ME, Ziegler CM, Russo J, Bruce EA, Ballif BA, Botten J. King BR, et al. Among authors: botten j. J Virol. 2017 Jul 12;91(15):e00763-17. doi: 10.1128/JVI.00763-17. Print 2017 Aug 1. J Virol. 2017. PMID: 28539447 Free PMC article.
Visualization of Arenavirus RNA Species in Individual Cells by Single-Molecule Fluorescence In Situ Hybridization Suggests a Model of Cyclical Infection and Clearance during Persistence.
King BR, Samacoits A, Eisenhauer PL, Ziegler CM, Bruce EA, Zenklusen D, Zimmer C, Mueller F, Botten J. King BR, et al. Among authors: botten j. J Virol. 2018 May 29;92(12):e02241-17. doi: 10.1128/JVI.02241-17. Print 2018 Jun 15. J Virol. 2018. PMID: 29643234 Free PMC article.
DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP.
Bruce EA, Huang ML, Perchetti GA, Tighe S, Laaguiby P, Hoffman JJ, Gerrard DL, Nalla AK, Wei Y, Greninger AL, Diehl SA, Shirley DJ, Leonard DGB, Huston CD, Kirkpatrick BD, Dragon JA, Crothers JW, Jerome KR, Botten JW. Bruce EA, et al. Among authors: botten jw. bioRxiv [Preprint]. 2020 Apr 6:2020.03.20.001008. doi: 10.1101/2020.03.20.001008. bioRxiv. 2020. PMID: 32511328 Free PMC article. Updated. Preprint.
Kinetics and Isotype Assessment of Antibodies Targeting the Spike Protein Receptor Binding Domain of SARS-CoV-2 In COVID-19 Patients as a function of Age and Biological Sex.
Graham NR, Whitaker AN, Strother CA, Miles AK, Grier D, McElvany BD, Bruce EA, Poynter ME, Pierce KK, Kirkpatrick BD, Stapleton RD, An G, Botten JW, Crothers JW, Diehl SA. Graham NR, et al. medRxiv [Preprint]. 2020 Jul 16:2020.07.15.20154443. doi: 10.1101/2020.07.15.20154443. medRxiv. 2020. PMID: 32743592 Free PMC article. Updated. Preprint.
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