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The nucleoid-associated DNA-binding protein H-NS is required for the efficient adaptation of Escherichia coli K-12 to a cold environment.
Dersch P, Kneip S, Bremer E. Dersch P, et al. Among authors: bremer e. Mol Gen Genet. 1994 Oct 28;245(2):255-9. doi: 10.1007/BF00283274. Mol Gen Genet. 1994. PMID: 7816034
We show here that the ability to cope efficiently with a cold environment (12 degrees C and 25 degrees C) is strongly impaired in E. coli strains carrying hns mutations. ...
We show here that the ability to cope efficiently with a cold environment (12 degrees C and 25 degrees C) is strongly impaired in E. …
Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.
von Blohn C, Kempf B, Kappes RM, Bremer E. von Blohn C, et al. Among authors: bremer e. Mol Microbiol. 1997 Jul;25(1):175-87. doi: 10.1046/j.1365-2958.1997.4441809.x. Mol Microbiol. 1997. PMID: 11902719
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Kappes RM, Kempf B, Kneip S, Boch J, Gade J, Meier-Wagner J, Bremer E. Kappes RM, et al. Among authors: bremer e. Mol Microbiol. 1999 Apr;32(1):203-16. doi: 10.1046/j.1365-2958.1999.01354.x. Mol Microbiol. 1999. PMID: 10216873
Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A- and sigma B-dependent stress-responsive promoters.
Spiegelhalter F, Bremer E. Spiegelhalter F, et al. Among authors: bremer e. Mol Microbiol. 1998 Jul;29(1):285-96. doi: 10.1046/j.1365-2958.1998.00929.x. Mol Microbiol. 1998. PMID: 9701821
A novel amidohydrolase gene from Bacillus subtilis cloning: DNA-sequence analysis and map position of amhX.
Kempf B, Bremer E. Kempf B, et al. Among authors: bremer e. FEMS Microbiol Lett. 1996 Aug 1;141(2-3):129-37. doi: 10.1111/j.1574-6968.1996.tb08374.x. FEMS Microbiol Lett. 1996. PMID: 8768514
The amhX gene was overexpressed in E. coli by using the T7 RNA polymerase/promoter system and the transcription initiation sites for the amhX mRNAs in B. subtilis were determined by primer extension analysis. ...
The amhX gene was overexpressed in E. coli by using the T7 RNA polymerase/promoter system and the transcription initiation sites for …
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
Boch J, Kempf B, Schmid R, Bremer E. Boch J, et al. Among authors: bremer e. J Bacteriol. 1996 Sep;178(17):5121-9. doi: 10.1128/jb.178.17.5121-5129.1996. J Bacteriol. 1996. PMID: 8752328 Free PMC article.
Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD.
Kappes RM, Kempf B, Bremer E. Kappes RM, et al. Among authors: bremer e. J Bacteriol. 1996 Sep;178(17):5071-9. doi: 10.1128/jb.178.17.5071-5079.1996. J Bacteriol. 1996. PMID: 8752321 Free PMC article.
The DNA sequence of a 2,310-bp segment of the cloned region revealed a single gene (opuD) whose product (OpuD) was essential for glycine betaine uptake and osmoprotection in E. coli. ...OpuD shows a significant degree of sequence identity to the choline transporter BetT an …
The DNA sequence of a 2,310-bp segment of the cloned region revealed a single gene (opuD) whose product (OpuD) was essential for glycine bet …
Synthesis of the Escherichia coli K-12 nucleoid-associated DNA-binding protein H-NS is subjected to growth-phase control and autoregulation.
Dersch P, Schmidt K, Bremer E. Dersch P, et al. Among authors: bremer e. Mol Microbiol. 1993 May;8(5):875-89. doi: 10.1111/j.1365-2958.1993.tb01634.x. Mol Microbiol. 1993. PMID: 8355613
Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli.
Dersch P, Fsihi H, Bremer E. Dersch P, et al. Among authors: bremer e. FEMS Microbiol Lett. 1994 Oct 15;123(1-2):19-26. doi: 10.1111/j.1574-6968.1994.tb07195.x. FEMS Microbiol Lett. 1994. PMID: 7988888
The pPD expression vectors were used to achieve high-level overproduction of the E. coli integral outer membrane protein Tsx, and the cytoplasmic enzymes beta-galactosidase (beta Gal) and UTP:alpha-D-glucose-1-phosphate uridylyltransferase (GalU). The characteristics of th …
The pPD expression vectors were used to achieve high-level overproduction of the E. coli integral outer membrane protein Tsx, and the …
pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli.
Herbst B, Kneip S, Bremer E. Herbst B, et al. Among authors: bremer e. Gene. 1994 Dec 30;151(1-2):137-42. doi: 10.1016/0378-1119(94)90644-0. Gene. 1994. PMID: 7828862
The properties of the pOSEX expression vectors were tested by positioning a promoterless lacZ (encoding beta-galactosidase) gene from E. coli and the gcdA (encoding carboxytransferase) gene from the Gram+ bacterium Acidaminococcus fermentans under the control of the proU r …
The properties of the pOSEX expression vectors were tested by positioning a promoterless lacZ (encoding beta-galactosidase) gene from E
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