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Isolation of lactose permease mutants which recognize arabinose.
Goswitz VC, Brooker RJ. Goswitz VC, et al. Membr Biochem. 1993 Jan-Mar;10(1):61-70. doi: 10.3109/09687689309150253. Membr Biochem. 1993. PMID: 8510563
Evidence that highly conserved residues of transmembrane segment 6 of Escherichia coli MntH are important for transport activity.
Haemig HA, Moen PJ, Brooker RJ. Haemig HA, et al. Biochemistry. 2010 Jun 8;49(22):4662-71. doi: 10.1021/bi100320y. Biochemistry. 2010. PMID: 20441230 Free PMC article.
., and Brooker, R. J. (2004) J. Membr. Biol. 201 (2), 97-107]. On the basis of site-directed mutagenesis of highly conserved negatively charged residues, a model was proposed for the metal binding site of the Escherichia coli homologue, MntH. ...
., and Brooker, R. J. (2004) J. Membr. Biol. 201 (2), 97-107]. On the basis of site-directed mutagenesis of highly conserved negative …
Evidence for structural symmetry and functional asymmetry in the lactose permease of Escherichia coli.
Green AL, Hrodey HA, Brooker RJ. Green AL, et al. Biochemistry. 2003 Sep 30;42(38):11226-33. doi: 10.1021/bi034810+. Biochemistry. 2003. PMID: 14503872
., and Brooker, R. J. [2001] Biochemistry 40, 12220-12229). Substitutions at positions 261, 265, 268, 272, and 276, which form a continuous stripe along TMS-8, were markedly defective for lactose transport velocity. ...
., and Brooker, R. J. [2001] Biochemistry 40, 12220-12229). Substitutions at positions 261, 265, 268, 272, and 276, which form a cont …
An isotope-edited FT-IR study of a symporter, the lactose permease.
Patzlaff JS, Zhang J, Brooker RJ, Barry BA. Patzlaff JS, et al. Biochemistry. 2002 Jun 11;41(23):7366-72. doi: 10.1021/bi025555g. Biochemistry. 2002. PMID: 12044169
., Brooker, R. J., and Barry, B. A. (2000) J. Biol. Chem. 275, 28695-28700]. In this study, we have globally labeled the permease with (13)C or (15)N. ...
., Brooker, R. J., and Barry, B. A. (2000) J. Biol. Chem. 275, 28695-28700]. In this study, we have globally labeled the permease wit …
Lactose permease mutants which transport (malto)-oligosaccharides.
Olsen SG, Greene KM, Brooker RJ. Olsen SG, et al. J Bacteriol. 1993 Oct;175(19):6269-75. doi: 10.1128/jb.175.19.6269-6275.1993. J Bacteriol. 1993. PMID: 8407799 Free PMC article.
A face on transmembrane segment 8 of the lactose permease is important for transport activity.
Green AL, Brooker RJ. Green AL, et al. Biochemistry. 2001 Oct 9;40(40):12220-9. doi: 10.1021/bi0109055. Biochemistry. 2001. PMID: 11580298
., and Brooker, R. J. (2000) J. Biol. Chem. 275, 23240-23246]. In the current study, mutagenesis was conducted along the side of predicted TMS-8 that contains the first amino acid in the conserved loop 8/9 motif. ...
., and Brooker, R. J. (2000) J. Biol. Chem. 275, 23240-23246]. In the current study, mutagenesis was conducted along the side of pred …
The conserved motif in hydrophilic loop 2/3 and loop 8/9 of the lactose permease of Escherichia coli. Analysis of suppressor mutations.
Cain SM, Matzke EA, Brooker RJ. Cain SM, et al. J Membr Biol. 2000 Jul 15;176(2):159-68. doi: 10.1007/s00232001085. J Membr Biol. 2000. PMID: 10926681
., & Brooker, R.J. 1996. J. Biol. Chem. 271:1400-1404; Jessen-Marshall, A.E., Parker, N., & Brooker, R.J. 1997. J. Bacteriol. 179:2616-2622; and Pazdernik, N., Cain, S.M., & Brooker, R.J. 1997. ...
., & Brooker, R.J. 1996. J. Biol. Chem. 271:1400-1404; Jessen-Marshall, A.E., Parker, N., & Brooker, R.J. 1997. J. Bac …
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