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Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms.
van Opijnen T, Bodi KL, Camilli A. van Opijnen T, et al. Nat Methods. 2009 Oct;6(10):767-72. doi: 10.1038/nmeth.1377. Epub 2009 Sep 20. Nat Methods. 2009. PMID: 19767758 Free PMC article.
Here we present a method (Tn-seq) for accurately determining quantitative genetic interactions on a genome-wide scale in microorganisms. ...Using this approach, we determined fitness for each gene of Streptococcus pneumoniae, a causative agent of pneumonia an …
Here we present a method (Tn-seq) for accurately determining quantitative genetic interactions on a genome-wide scale in micro …
Use of genetic recombination as a reporter of gene expression.
Camilli A, Beattie DT, Mekalanos JJ. Camilli A, et al. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2634-8. doi: 10.1073/pnas.91.7.2634. Proc Natl Acad Sci U S A. 1994. PMID: 8146167 Free PMC article.
To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette that permits the generation of transcriptional fusions to tnpR encoding resolvase, a site-specific recombinase of the transposable ele …
To extend the utility of the reporter gene fusion approach to such studies, we have constructed a gene expression reporter cassette t …
Nucleotide sequence and spatiotemporal expression of the Vibrio cholerae vieSAB genes during infection.
Lee SH, Angelichio MJ, Mekalanos JJ, Camilli A. Lee SH, et al. J Bacteriol. 1998 May;180(9):2298-305. doi: 10.1128/JB.180.9.2298-2305.1998. J Bacteriol. 1998. PMID: 9573178 Free PMC article.
The iviVII gene of Vibrio cholerae was previously identified by a screen for genes induced during intestinal infection. In the present study, nucleotide sequence analysis revealed that iviVII is a 1,659-bp open reading frame, herein designated vieB, that is predicte …
The iviVII gene of Vibrio cholerae was previously identified by a screen for genes induced during intestinal infection. In the presen …
Systematic identification of essential genes by in vitro mariner mutagenesis.
Akerley BJ, Rubin EJ, Camilli A, Lampe DJ, Robertson HM, Mekalanos JJ. Akerley BJ, et al. Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8927-32. doi: 10.1073/pnas.95.15.8927. Proc Natl Acad Sci U S A. 1998. PMID: 9671781 Free PMC article.
Here, we describe a simple system for performing transposon mutagenesis on naturally transformable organisms along with a technique to rapidly identify essential or conditionally essential DNA segments. ...
Here, we describe a simple system for performing transposon mutagenesis on naturally transformable organisms along with a tech …
Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae.
Bricker AL, Camilli A. Bricker AL, et al. FEMS Microbiol Lett. 1999 Mar 15;172(2):131-5. doi: 10.1111/j.1574-6968.1999.tb13460.x. FEMS Microbiol Lett. 1999. PMID: 10188240
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. ...An optimized protocol was developed whereby S. pneumoniae str …
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has …
Random transposon mutagenesis of Campylobacter jejuni.
Golden NJ, Camilli A, Acheson DW. Golden NJ, et al. Infect Immun. 2000 Sep;68(9):5450-3. doi: 10.1128/iai.68.9.5450-5453.2000. Infect Immun. 2000. PMID: 10948182 Free PMC article.
Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. ...
Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a
The ToxR-mediated organic acid tolerance response of Vibrio cholerae requires OmpU.
Merrell DS, Bailey C, Kaper JB, Camilli A. Merrell DS, et al. J Bacteriol. 2001 May;183(9):2746-54. doi: 10.1128/JB.183.9.2746-2754.2001. J Bacteriol. 2001. PMID: 11292792 Free PMC article.
The transcriptional regulator ToxR, while not involved in inorganic ATR, is required for organic ATR in a ToxT-independent manner. ...Characterization of the effect of organic acid stress on ompU and ompT transcription reveals that while ompU transcription remains virtuall …
The transcriptional regulator ToxR, while not involved in inorganic ATR, is required for organic ATR in a ToxT-independent manner. .. …
Identification of novel factors involved in colonization and acid tolerance of Vibrio cholerae.
Merrell DS, Hava DL, Camilli A. Merrell DS, et al. Mol Microbiol. 2002 Mar;43(6):1471-91. doi: 10.1046/j.1365-2958.2002.02857.x. Mol Microbiol. 2002. PMID: 11952899
A total of 164 mutants representing transposition events into 95 different open reading frames were shown to be recovered at greatly reduced numbers from the infant mouse model. ...Competition assays of these nine acid tolerance response (ATR)-defective mutants revealed th
A total of 164 mutants representing transposition events into 95 different open reading frames were shown to be recovered at greatly
Host-induced epidemic spread of the cholera bacterium.
Merrell DS, Butler SM, Qadri F, Dolganov NA, Alam A, Cohen MB, Calderwood SB, Schoolnik GK, Camilli A. Merrell DS, et al. Nature. 2002 Jun 6;417(6889):642-5. doi: 10.1038/nature00778. Nature. 2002. PMID: 12050664 Free PMC article.
It is unclear, however, whether stomach acidity is a principal factor contributing to epidemic spread. Here we report that characterization of Vibrio cholerae from human stools supports a model whereby human colonization creates a hyperinfectious bacterial st …
It is unclear, however, whether stomach acidity is a principal factor contributing to epidemic spread. Here we report that characteri …
The Vibrio cholerae vieSAB locus encodes a pathway contributing to cholera toxin production.
Tischler AD, Lee SH, Camilli A. Tischler AD, et al. J Bacteriol. 2002 Aug;184(15):4104-13. doi: 10.1128/jb.184.15.4104-4113.2002. J Bacteriol. 2002. PMID: 12107127 Free PMC article.
The genes encoding cholera toxin (CT), ctxAB, are coregulated with those for other Vibrio cholerae virulence factors by a cascade of transcriptional activators, including ToxR, TcpP, and ToxT. Additional regulators that modulate expression of ctxAB during infection were re …
The genes encoding cholera toxin (CT), ctxAB, are coregulated with those for other Vibrio cholerae virulence factors by a cascade of …
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