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Removal of DnaK contamination during fusion protein purifications.
Rial DV, Ceccarelli EA. Rial DV, et al. Among authors: ceccarelli ea. Protein Expr Purif. 2002 Aug;25(3):503-7. doi: 10.1016/s1046-5928(02)00024-4. Protein Expr Purif. 2002. PMID: 12182832
Assembly of plant ferredoxin-NADP+ oxidoreductase in Escherichia coli requires GroE molecular chaperones.
Carrillo N, Ceccarelli EA, Krapp AR, Boggio S, Ferreyra RG, Viale AM. Carrillo N, et al. Among authors: ceccarelli ea. J Biol Chem. 1992 Aug 5;267(22):15537-41. J Biol Chem. 1992. PMID: 1353496
We have recently reported the expression in Escherichia coli of an enzymatically competent ferredoxin-NADP+ oxidoreductase from cloned pea genes encoding either the mature enzyme or its precursor protein (Ceccarelli, E. ...
We have recently reported the expression in Escherichia coli of an enzymatically competent ferredoxin-NADP+ oxidoreductase from cloned pea g …
Expression, assembly, and processing of an active plant ferredoxin-NADP+ oxidoreductase and its precursor protein in Escherichia coli.
Ceccarelli EA, Viale AM, Krapp AR, Carrillo N. Ceccarelli EA, et al. J Biol Chem. 1991 Aug 5;266(22):14283-7. J Biol Chem. 1991. PMID: 1907276
The precursor of pea ferredoxin-NADP+ reductase synthesized in Escherichia coli contains bound FAD and is transported into chloroplasts.
Serra EC, Krapp AR, Ottado J, Feldman MF, Ceccarelli EA, Carrillo N. Serra EC, et al. Among authors: ceccarelli ea. J Biol Chem. 1995 Aug 25;270(34):19930-5. doi: 10.1074/jbc.270.34.19930. J Biol Chem. 1995. PMID: 7650008
One-step purification of plant ferredoxin-NADP+ oxidoreductase expressed in Escherichia coli as fusion with glutathione S-transferase.
Serra EC, Carrillo N, Krapp AR, Ceccarelli EA. Serra EC, et al. Among authors: ceccarelli ea. Protein Expr Purif. 1993 Dec;4(6):539-46. doi: 10.1006/prep.1993.1071. Protein Expr Purif. 1993. PMID: 8286951
Probing the role of the carboxyl-terminal region of ferredoxin-NADP+ reductase by site-directed mutagenesis and deletion analysis.
Orellano EG, Calcaterra NB, Carrillo N, Ceccarelli EA. Orellano EG, et al. Among authors: ceccarelli ea. J Biol Chem. 1993 Sep 15;268(26):19267-73. J Biol Chem. 1993. PMID: 8366077
Conformational requirements of a recombinant ferredoxin-NADP+ reductase precursor for efficient binding to and import into isolated chloroplasts.
Ceccarelli EA, Krapp AR, Serra EC, Carrillo N. Ceccarelli EA, et al. Eur J Biochem. 1996 May 15;238(1):192-7. doi: 10.1111/j.1432-1033.1996.0192q.x. Eur J Biochem. 1996. PMID: 8665937
Cooperation of the DnaK and GroE chaperone systems in the folding pathway of plant ferredoxin-NADP+ reductase expressed in Escherichia coli.
Dionisi HM, Checa SK, Krapp AR, Arakaki AK, Ceccarelli EA, Carrillo N, Viale AM. Dionisi HM, et al. Among authors: ceccarelli ea. Eur J Biochem. 1998 Feb 1;251(3):724-8. doi: 10.1046/j.1432-1327.1998.2510724.x. Eur J Biochem. 1998. PMID: 9490045
., Ceccarelli, E. A., Krapp, A. R., Boggio, S., Ferreyra, R. G. & Viale, A. M. (1992) J. Biol. Chem. 267, 15537-15541], the DnaK chaperone system plays a crucial role in the folding pathway of FNR....
., Ceccarelli, E. A., Krapp, A. R., Boggio, S., Ferreyra, R. G. & Viale, A. M. (1992) J. Biol. Chem. 267, 15537-15541], the DnaK …
A fully active FAD-containing precursor remains folded up to its translocation across the chloroplast membranes.
Ottado J, Ceccarelli EA. Ottado J, et al. Among authors: ceccarelli ea. Eur J Biochem. 1998 Apr 1;253(1):132-8. doi: 10.1046/j.1432-1327.1998.2530132.x. Eur J Biochem. 1998. PMID: 9578470
Spectroscopic characterization of a binuclear metal site in Bacillus cereus beta-lactamase II.
Orellano EG, Girardini JE, Cricco JA, Ceccarelli EA, Vila AJ. Orellano EG, et al. Among authors: ceccarelli ea. Biochemistry. 1998 Jul 14;37(28):10173-80. doi: 10.1021/bi980309j. Biochemistry. 1998. PMID: 9665723
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