Centaurea species are widely used in traditional medicine to treat several illnesses, especially by Mediterranean populations due to their pharmacological properties. The present study aimed to evaluate for the first time some in vivo activities of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis. For this approach; the antidiabetic (streptozotocin-induced diabetes), hepatoprotective (paracetamol induced hepatotoxicity), anti-inflammatory (croton oil induced ear edema assay) and antipyretic activities of this plant extract were tested. The pharmacological results suggest that C. tougourensis has a non-negligible anti-inflammatory effect on the formation of ear edema with a maximum inhibition percentage of (39.58%) for the highest tested concentration of 400 mg/kg. However, the antipyretic activity of the plant was remarkable for both tested concentrations (200 and 400 mg/kg) 5 h after treatment with a significant (P < 0.05) reduction of rectal temperature to (32.88 ± 0.23°C) and (32.36 ± 0.18°C) which correspond to a pyrexia inhibition of (78.9%) and (90.18%) respectively. C. tougourensis exhibited also a good anti-hyperglycemic effect which reached an inhibition percentage of (68.29%) at the end of the 3rd week of treatment for the tested concentration of 400 mg/kg and was considered almost similar to those of standard value (71.83%) at the same time. The n-BuOH extract C. tougourensis showed also a remarkable hepatoprotective effect which was confirmed by biochemical and histological approaches of note is that natural silymarin was also used as reference drug and showed a remarkable hepatoprotective effect. These encouraging results demonstrated once again the pharmacological potential of Centaurea species.