Abstract
The rhizobacterium Pseudomonas brassicacearum forms phenotypic variants which do not show extracellular protease and lipase activity. The operon encoding these enzymes, a serine protease homolog, and a type I secretion machinery was characterized. Transcriptional lacZ gene fusions revealed that the expression of the operon is under the control of phase variation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cosmids
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Gene Expression Regulation, Bacterial
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Lipase / chemistry
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Lipase / genetics*
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Lipase / metabolism
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Membrane Proteins*
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Membrane Transport Proteins*
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Molecular Sequence Data
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Operon*
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Promoter Regions, Genetic
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Pseudomonas / enzymology
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Pseudomonas / genetics*
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Pseudomonas / physiology
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Recombinant Proteins / metabolism
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Sequence Analysis, DNA
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Subtilisins / chemistry
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Subtilisins / genetics*
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Subtilisins / metabolism
Substances
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ATP-Binding Cassette Transporters
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AprD protein, Pseudomonas fluorescens
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AprE protein, Bacteria
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AprF protein, Pseudomonas
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Bacterial Proteins
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LipA protein, Bacteria
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Membrane Proteins
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Membrane Transport Proteins
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Recombinant Proteins
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Lipase
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Subtilisins