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Development of a fed-batch fermentation process to overproduce phosphoenolpyruvate carboxykinase using an expression vector with promoter and plasmid copy number controllable by heat.
Chao YP, Chern JT, Lin WS, Wang ZW. Chao YP, et al. Biotechnol Bioeng. 2003 Nov 20;84(4):459-66. doi: 10.1002/bit.10796. Biotechnol Bioeng. 2003. PMID: 14574704
Improvement of the thermoregulated T7 expression system by using the heat-sensitive lacI.
Wang ZW, Law WS, Chao YP. Wang ZW, et al. Biotechnol Prog. 2004 Sep-Oct;20(5):1352-8. doi: 10.1021/bp049851r. Biotechnol Prog. 2004. PMID: 15458317
The thermoregulated T7 expression system was previously reported to be an effective way to produce massive amounts of recombinant proteins (Chao, Y. ...
The thermoregulated T7 expression system was previously reported to be an effective way to produce massive amounts of recombinant proteins ( …
Chitin-binding domain based immobilization of D-hydantoinase.
Chern JT, Chao YP. Chern JT, et al. J Biotechnol. 2005 May 25;117(3):267-75. doi: 10.1016/j.jbiotec.2005.02.001. Epub 2005 Mar 25. J Biotechnol. 2005. PMID: 15862357
Immobilization of cells with surface-displayed chitin-binding domain.
Wang JY, Chao YP. Wang JY, et al. Appl Environ Microbiol. 2006 Jan;72(1):927-31. doi: 10.1128/AEM.72.1.927-931.2006. Appl Environ Microbiol. 2006. PMID: 16391137 Free PMC article.
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