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An improved PCR method for walking in uncloned genomic DNA.
Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA. Siebert PD, et al. Among authors: chenchik a. Nucleic Acids Res. 1995 Mar 25;23(6):1087-8. doi: 10.1093/nar/23.6.1087. Nucleic Acids Res. 1995. PMID: 7731798 Free PMC article.
Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA.
Diachenko LB, Ledesma J, Chenchik AA, Siebert PD. Diachenko LB, et al. Among authors: chenchik aa. Biochem Biophys Res Commun. 1996 Feb 27;219(3):824-8. doi: 10.1006/bbrc.1996.0317. Biochem Biophys Res Commun. 1996. PMID: 8645264
Our protocol requires only a single cDNA synthesis for each different RNA sample, in contrast to the multiple cDNA reactions required for differential display method, followed by selective amplification of cDNA sequence fraction by arbitrary and oligo(dT) primers. ...Long- …
Our protocol requires only a single cDNA synthesis for each different RNA sample, in contrast to the multiple cDNA reactions required …
Nylon cDNA expression arrays.
Jokhadze G, Chen S, Granger C, Chenchik A. Jokhadze G, et al. Among authors: chenchik a. Methods Mol Biol. 2003;224:9-29. doi: 10.1385/1-59259-364-X:09. Methods Mol Biol. 2003. PMID: 12710663 No abstract available.
Amplification of cDNA ends based on template-switching effect and step-out PCR.
Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A. Matz M, et al. Among authors: chenchik a. Nucleic Acids Res. 1999 Mar 15;27(6):1558-60. doi: 10.1093/nar/27.6.1558. Nucleic Acids Res. 1999. PMID: 10037822 Free PMC article.
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generat
Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction.
Zhu YY, Machleder EM, Chenchik A, Li R, Siebert PD. Zhu YY, et al. Among authors: chenchik a. Biotechniques. 2001 Apr;30(4):892-7. doi: 10.2144/01304pf02. Biotechniques. 2001. PMID: 11314272
Following reverse transcription, three cycles of PCR are performed using a modified oligo(dT) primer and an anchor primer to enrich the cDNA population for full-length sequences. Starting with 1 microgram human skeletal muscle poly(A)+ RNA, a cDNA library was …
Following reverse transcription, three cycles of PCR are performed using a modified oligo(dT) primer and an anchor primer to enrich t …
Use of SMART-generated cDNA for gene expression studies in multiple human tumors.
Zhumabayeva B, Diatchenko L, Chenchik A, Siebert PD. Zhumabayeva B, et al. Among authors: chenchik a. Biotechniques. 2001 Jan;30(1):158-63. doi: 10.2144/01301pf01. Biotechniques. 2001. PMID: 11196307
We demonstrate here that SMART PCR-amplified cDNAs arrayed on a nylon membrane are suitable for high-throughput tissue expression profiling when starting biological materials are limited. ...We also arrayed cDNAs from 68 matched tumor and normal samples on a nylon m …
We demonstrate here that SMART PCR-amplified cDNAs arrayed on a nylon membrane are suitable for high-throughput tissue expression pro …
RecA-mediated affinity capture: a method for full-length cDNA cloning.
Zhumabayeva B, Chenchik A, Siebert PD. Zhumabayeva B, et al. Among authors: chenchik a. Biotechniques. 1999 Oct;27(4):834-6, 838, 840 passim. doi: 10.2144/99274rr06. Biotechniques. 1999. PMID: 10524326
This approach is based on the ability of Escherichia coli RecA protein to form a stable nucleoprotein complex with a linear single-stranded DNA probe and homologous sequences in circular double-stranded DNA. ...Typically, many clones can then be recovered by colony …
This approach is based on the ability of Escherichia coli RecA protein to form a stable nucleoprotein complex with a linear si …
Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplification of adaptor-ligated cDNA.
Chenchik A, Diachenko L, Moqadam F, Tarabykin V, Lukyanov S, Siebert PD. Chenchik A, et al. Biotechniques. 1996 Sep;21(3):526-34. doi: 10.2144/96213pf02. Biotechniques. 1996. PMID: 8879595
In this approach, a double-stranded (ds) adaptor is ligated to both ends of a library of ds cDNA by T4 DNA ligase. This adaptor-ligated ds cDNA is then used to selectively amplify 5'- or 3'-cDNA fragments by PCR with a combination of gene-specific and adaptor …
In this approach, a double-stranded (ds) adaptor is ligated to both ends of a library of ds cDNA by T4 DNA ligase. This adapto …
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD. Diatchenko L, et al. Among authors: chenchik a. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30. doi: 10.1073/pnas.93.12.6025. Proc Natl Acad Sci U S A. 1996. PMID: 8650213 Free PMC article.
It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. ...In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hy …
It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a
TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase.
Kellogg DE, Rybalkin I, Chen S, Mukhamedova N, Vlasik T, Siebert PD, Chenchik A. Kellogg DE, et al. Among authors: chenchik a. Biotechniques. 1994 Jun;16(6):1134-7. Biotechniques. 1994. PMID: 8074881
The MAbs, incubated with Taq DNA polymerase and added to PCR tubes at ambient temperature, yield specific DNA fragments upon amplification when using high numbers of temperature cycles and a very low copy number of target DNA in a complex DNA background. ...
The MAbs, incubated with Taq DNA polymerase and added to PCR tubes at ambient temperature, yield specific DNA fragments upon amplification w …
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