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Assembly of large, high G+C bacterial DNA fragments in yeast.
Noskov VN, Karas BJ, Young L, Chuang RY, Gibson DG, Lin YC, Stam J, Yonemoto IT, Suzuki Y, Andrews-Pfannkoch C, Glass JI, Smith HO, Hutchison CA 3rd, Venter JC, Weyman PD. Noskov VN, et al. Among authors: chuang ry. ACS Synth Biol. 2012 Jul 20;1(7):267-73. doi: 10.1021/sb3000194. Epub 2012 May 21. ACS Synth Biol. 2012. PMID: 23651249
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI. Lartigue C, et al. Among authors: chuang ry. Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20. Science. 2009. PMID: 19696314
Cloning whole bacterial genomes in yeast.
Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA 3rd. Benders GA, et al. Among authors: chuang ry. Nucleic Acids Res. 2010 May;38(8):2558-69. doi: 10.1093/nar/gkq119. Epub 2010 Mar 7. Nucleic Acids Res. 2010. PMID: 20211840 Free PMC article.
Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC. Gibson DG, et al. Among authors: chuang ry. Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20. Science. 2010. PMID: 20488990
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.
Tagwerker C, Dupont CL, Karas BJ, Ma L, Chuang RY, Benders GA, Ramon A, Novotny M, Montague MG, Venepally P, Brami D, Schwartz A, Andrews-Pfannkoch C, Gibson DG, Glass JI, Smith HO, Venter JC, Hutchison CA 3rd. Tagwerker C, et al. Among authors: chuang ry. Nucleic Acids Res. 2012 Nov 1;40(20):10375-83. doi: 10.1093/nar/gks823. Epub 2012 Aug 31. Nucleic Acids Res. 2012. PMID: 22941652 Free PMC article.
Assembly of eukaryotic algal chromosomes in yeast.
Karas BJ, Molparia B, Jablanovic J, Hermann WJ, Lin YC, Dupont CL, Tagwerker C, Yonemoto IT, Noskov VN, Chuang RY, Allen AE, Glass JI, Hutchison CA 3rd, Smith HO, Venter JC, Weyman PD. Karas BJ, et al. Among authors: chuang ry. J Biol Eng. 2013 Dec 10;7(1):30. doi: 10.1186/1754-1611-7-30. J Biol Eng. 2013. PMID: 24325901 Free PMC article.
Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.
Suzuki Y, Assad-Garcia N, Kostylev M, Noskov VN, Wise KS, Karas BJ, Stam J, Montague MG, Hanly TJ, Enriquez NJ, Ramon A, Goldgof GM, Richter RA, Vashee S, Chuang RY, Winzeler EA, Hutchison CA 3rd, Gibson DG, Smith HO, Glass JI, Venter JC. Suzuki Y, et al. Among authors: chuang ry. Genome Res. 2015 Mar;25(3):435-44. doi: 10.1101/gr.182477.114. Epub 2015 Feb 5. Genome Res. 2015. PMID: 25654978 Free PMC article.
Design and synthesis of a minimal bacterial genome.
Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC. Hutchison CA 3rd, et al. Among authors: chuang ry. Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253. Science. 2016. PMID: 27013737
112 results