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Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches.
Bratovič M, Fonfara I, Chylinski K, Gálvez EJC, Sullivan TJ, Boerno S, Timmermann B, Boettcher M, Charpentier E. Bratovič M, et al. Among authors: chylinski k. Nat Chem Biol. 2020 May;16(5):587-595. doi: 10.1038/s41589-020-0490-4. Epub 2020 Mar 2. Nat Chem Biol. 2020. PMID: 32123387
Classification and evolution of type II CRISPR-Cas systems.
Chylinski K, Makarova KS, Charpentier E, Koonin EV. Chylinski K, et al. Nucleic Acids Res. 2014 Jun;42(10):6091-105. doi: 10.1093/nar/gku241. Epub 2014 Apr 11. Nucleic Acids Res. 2014. PMID: 24728998 Free PMC article. Review.
A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo.
Bestas B, Wimberger S, Degtev D, Madsen A, Rottner AK, Karlsson F, Naumenko S, Callahan M, Touza JL, Francescatto M, Möller CI, Badertscher L, Li S, Cerboni S, Selfjord N, Ericson E, Gordon E, Firth M, Chylinski K, Taheri-Ghahfarokhi A, Bohlooly-Y M, Snowden M, Pangalos M, Nuttall B, Akcakaya P, Sienski G, Maresca M. Bestas B, et al. Among authors: chylinski k. Nat Commun. 2023 Sep 6;14(1):5474. doi: 10.1038/s41467-023-41240-7. Nat Commun. 2023. PMID: 37673883 Free PMC article.