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Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site.
Tomic TI, Moric I, Conn GL, Vasiljevic B. Tomic TI, et al. Res Microbiol. 2008 Nov-Dec;159(9-10):658-62. doi: 10.1016/j.resmic.2008.09.006. Epub 2008 Oct 1. Res Microbiol. 2008. PMID: 18930134 Free PMC article.
Expansion of the aminoglycoside-resistance 16S rRNA (m(1)A1408) methyltransferase family: expression and functional characterization of four hypothetical enzymes of diverse bacterial origin.
Witek MA, Conn GL. Witek MA, et al. Biochim Biophys Acta. 2014 Sep;1844(9):1648-55. doi: 10.1016/j.bbapap.2014.06.012. Epub 2014 Jun 22. Biochim Biophys Acta. 2014. PMID: 24963996 Free PMC article.
Expression, purification and crystallization of adenosine 1408 aminoglycoside-resistance rRNA methyltransferases for structural studies.
Zelinskaya N, Rankin CR, Macmaster R, Savic M, Conn GL. Zelinskaya N, et al. Protein Expr Purif. 2011 Jan;75(1):89-94. doi: 10.1016/j.pep.2010.07.005. Epub 2010 Jul 25. Protein Expr Purif. 2011. PMID: 20667473 Free PMC article.
Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria.
Macmaster R, Zelinskaya N, Savic M, Rankin CR, Conn GL. Macmaster R, et al. Nucleic Acids Res. 2010 Nov;38(21):7791-9. doi: 10.1093/nar/gkq627. Epub 2010 Jul 17. Nucleic Acids Res. 2010. PMID: 20639535 Free PMC article.
Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics.
Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL. Savic M, et al. Nucleic Acids Res. 2009 Sep;37(16):5420-31. doi: 10.1093/nar/gkp575. Epub 2009 Jul 9. Nucleic Acids Res. 2009. PMID: 19589804 Free PMC article.
30S Subunit-dependent activation of the Sorangium cellulosum So ce56 aminoglycoside resistance-conferring 16S rRNA methyltransferase Kmr.
Savic M, Sunita S, Zelinskaya N, Desai PM, Macmaster R, Vinal K, Conn GL. Savic M, et al. Antimicrob Agents Chemother. 2015 May;59(5):2807-16. doi: 10.1128/AAC.00056-15. Epub 2015 Mar 2. Antimicrob Agents Chemother. 2015. PMID: 25733511 Free PMC article.
The Pathogen-Derived Aminoglycoside Resistance 16S rRNA Methyltransferase NpmA Possesses Dual m1A1408/m1G1408 Specificity.
Zelinskaya N, Witek MA, Conn GL. Zelinskaya N, et al. Antimicrob Agents Chemother. 2015 Dec;59(12):7862-5. doi: 10.1128/AAC.01872-15. Epub 2015 Sep 28. Antimicrob Agents Chemother. 2015. PMID: 26416864 Free PMC article.
Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm.
Savic M, Ilic-Tomic T, Macmaster R, Vasiljevic B, Conn GL. Savic M, et al. J Bacteriol. 2008 Sep;190(17):5855-61. doi: 10.1128/JB.00076-08. Epub 2008 Jun 27. J Bacteriol. 2008. PMID: 18586937 Free PMC article.
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