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Cryo-electron microscopy of chromatin biology.
Wilson MD, Costa A. Wilson MD, et al. Acta Crystallogr D Struct Biol. 2017 Jun 1;73(Pt 6):541-548. doi: 10.1107/S2059798317004430. Epub 2017 Apr 20. Acta Crystallogr D Struct Biol. 2017. PMID: 28580916 Free PMC article. Review.
Recent electron-microscopy (EM) studies on NCP-containing assemblies have helped to describe important chromatin transactions at a molecular level. With the implementation of recent technical advances in single-particle EM, our understanding of how nucleosomes are recogniz …
Recent electron-microscopy (EM) studies on NCP-containing assemblies have helped to describe important chromatin transactions at a mo …
Structural studies of the archaeal MCM complex in different functional states.
Costa A, Pape T, van Heel M, Brick P, Patwardhan A, Onesti S. Costa A, et al. J Struct Biol. 2006 Oct;156(1):210-9. doi: 10.1016/j.jsb.2006.04.001. Epub 2006 May 2. J Struct Biol. 2006. PMID: 16731005
The crystal structure of the DNA-interacting N-terminal domain of this homo-oligomer revealed a double hexamer in a head-to-head configuration; single-particle electron microscopy studies have shown that the full-length protein complex can form both single and doubl …
The crystal structure of the DNA-interacting N-terminal domain of this homo-oligomer revealed a double hexamer in a head-to-he …
A novel mirror-symmetry analysis approach for the study of macromolecular assemblies imaged by electron microscopy.
Costa A, Patwardhan A. Costa A, et al. J Mol Biol. 2008 Apr 18;378(1):273-83. doi: 10.1016/j.jmb.2008.02.054. Epub 2008 Mar 4. J Mol Biol. 2008. PMID: 18353361
Multivariate statistical symmetry analysis is widely employed in single-particle electron-microscopy studies for the detection of symmetry components within a set of noisy two-dimensional images. ...Here, we propose a method to detect symmetry components from side- …
Multivariate statistical symmetry analysis is widely employed in single-particle electron-microscopy studies for the detection of symmetry c …
Cryo-electron microscopy reveals a novel DNA-binding site on the MCM helicase.
Costa A, van Duinen G, Medagli B, Chong J, Sakakibara N, Kelman Z, Nair SK, Patwardhan A, Onesti S. Costa A, et al. EMBO J. 2008 Aug 20;27(16):2250-8. doi: 10.1038/emboj.2008.135. Epub 2008 Jul 24. EMBO J. 2008. PMID: 18650940 Free PMC article.
To characterize the interplay between the MCM helicase and DNA prior to the melting of the double helix, we determined the structure of an archaeal MCM orthologue bound to a 5.6-kb double-stranded DNA segment, using cryo-electron microscopy. DNA wraps around the N-terminal …
To characterize the interplay between the MCM helicase and DNA prior to the melting of the double helix, we determined the structure of an a …
Structural basis for retroviral integration into nucleosomes.
Maskell DP, Renault L, Serrao E, Lesbats P, Matadeen R, Hare S, Lindemann D, Engelman AN, Costa A, Cherepanov P. Maskell DP, et al. Nature. 2015 Jul 16;523(7560):366-9. doi: 10.1038/nature14495. Epub 2015 Jun 10. Nature. 2015. PMID: 26061770 Free PMC article.
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome. ...Single-particle cryo-electron microscopy reveals a multivalent intasome-nucleosome interface involving both gyres of …
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as …
Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader.
Chao WC, Murayama Y, Muñoz S, Costa A, Uhlmann F, Singleton MR. Chao WC, et al. Cell Rep. 2015 Aug 4;12(5):719-25. doi: 10.1016/j.celrep.2015.06.071. Epub 2015 Jul 23. Cell Rep. 2015. PMID: 26212329
Here, we report the crystal structure of Scc4 bound to the N terminus of Scc2 and show that Scc4 is a tetratricopeptide repeat (TPR) superhelix. ...Electron microscopy (EM) analysis reveals that the Scc2-Scc4 loader complex comprises three domains: a head, body, and …
Here, we report the crystal structure of Scc4 bound to the N terminus of Scc2 and show that Scc4 is a tetratricopeptide repeat (TPR) …
The MCM Helicase Motor of the Eukaryotic Replisome.
Abid Ali F, Costa A. Abid Ali F, et al. J Mol Biol. 2016 May 8;428(9 Pt B):1822-32. doi: 10.1016/j.jmb.2016.01.024. Epub 2016 Jan 30. J Mol Biol. 2016. PMID: 26829220 Review.
The MCM motor of the CMG helicase powers ahead of the eukaryotic replication machinery to unwind DNA, in a process that requires ATP hydrolysis. ...Despite the exciting progress, how the MCM translocates on DNA remains a matter of debate....
The MCM motor of the CMG helicase powers ahead of the eukaryotic replication machinery to unwind DNA, in a process that requires ATP …
Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate.
Abid Ali F, Renault L, Gannon J, Gahlon HL, Kotecha A, Zhou JC, Rueda D, Costa A. Abid Ali F, et al. Nat Commun. 2016 Feb 18;7:10708. doi: 10.1038/ncomms10708. Nat Commun. 2016. PMID: 26888060 Free PMC article.
Here we use cryo-electron microscopy to describe two subnanometre resolution structures of the CMG helicase trapped on a DNA fork. In the predominant state, the ring-shaped C-terminal ATPase of MCM is compact and contacts single-stranded DNA, via a set of pre-sensor …
Here we use cryo-electron microscopy to describe two subnanometre resolution structures of the CMG helicase trapped on a DNA fork. In …
Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome.
Samora CP, Saksouk J, Goswami P, Wade BO, Singleton MR, Bates PA, Lengronne A, Costa A, Uhlmann F. Samora CP, et al. Mol Cell. 2016 Aug 4;63(3):371-84. doi: 10.1016/j.molcel.2016.05.036. Epub 2016 Jul 7. Mol Cell. 2016. PMID: 27397686 Free PMC article.
Here we show that Ctf4 recruits the Chl1 helicase to the replisome via a conserved interaction motif that Chl1 shares with GINS and polymerase α. ...A physical interaction between Chl1 and the cohesin complex during S phase suggests that Chl1 contacts cohesin to fac …
Here we show that Ctf4 recruits the Chl1 helicase to the replisome via a conserved interaction motif that Chl1 shares with GINS and p …
New Insights into the Mechanism of DNA Duplication by the Eukaryotic Replisome.
Pellegrini L, Costa A. Pellegrini L, et al. Trends Biochem Sci. 2016 Oct;41(10):859-871. doi: 10.1016/j.tibs.2016.07.011. Epub 2016 Aug 20. Trends Biochem Sci. 2016. PMID: 27555051 Review.
The DNA replication machinery, or replisome, is a macromolecular complex that combines DNA unwinding, priming and synthesis activities. ...
The DNA replication machinery, or replisome, is a macromolecular complex that combines DNA unwinding, priming and synthesis activitie …
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