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Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast.
Nagar G, Jain S, Rajurkar M, Lothe R, Rao H, Majumdar S, Gautam M, Rodriguez-Aponte SA, Crowell LE, Love JC, Dandekar P, Puranik A, Gairola S, Shaligram U, Jain R. Nagar G, et al. Among authors: crowell le. Vaccines (Basel). 2023 Oct 16;11(10):1602. doi: 10.3390/vaccines11101602. Vaccines (Basel). 2023. PMID: 37897004 Free PMC article.
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Dalvie NC, Biedermann AM, Rodriguez-Aponte SA, Naranjo CA, Rao HD, Rajurkar MP, Lothe RR, Shaligram US, Johnston RS, Crowell LE, Castelino S, Tracey MK, Whittaker CA, Love JC. Dalvie NC, et al. Among authors: crowell le. Biotechnol Bioeng. 2022 Feb;119(2):657-662. doi: 10.1002/bit.27979. Epub 2021 Nov 15. Biotechnol Bioeng. 2022. PMID: 34780057 Free PMC article.
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice.
Dalvie NC, Rodriguez-Aponte SA, Hartwell BL, Tostanoski LH, Biedermann AM, Crowell LE, Kaur K, Kumru OS, Carter L, Yu J, Chang A, McMahan K, Courant T, Lebas C, Lemnios AA, Rodrigues KA, Silva M, Johnston RS, Naranjo CA, Tracey MK, Brady JR, Whittaker CA, Yun D, Brunette N, Wang JY, Walkey C, Fiala B, Kar S, Porto M, Lok M, Andersen H, Lewis MG, Love KR, Camp DL, Silverman JM, Kleanthous H, Joshi SB, Volkin DB, Dubois PM, Collin N, King NP, Barouch DH, Irvine DJ, Love JC. Dalvie NC, et al. Among authors: crowell le. Proc Natl Acad Sci U S A. 2021 Sep 21;118(38):e2106845118. doi: 10.1073/pnas.2106845118. Proc Natl Acad Sci U S A. 2021. PMID: 34493582 Free PMC article.
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus.
Dalvie NC, Brady JR, Crowell LE, Tracey MK, Biedermann AM, Kaur K, Hickey JM, Kristensen DL 2nd, Bonnyman AD, Rodriguez-Aponte SA, Whittaker CA, Bok M, Vega C, Mukhopadhyay TK, Joshi SB, Volkin DB, Parreño V, Love KR, Love JC. Dalvie NC, et al. Among authors: crowell le. Microb Cell Fact. 2021 May 1;20(1):94. doi: 10.1186/s12934-021-01583-6. Microb Cell Fact. 2021. PMID: 33933073 Free PMC article.
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Dalvie NC, Biedermann AM, Rodriguez-Aponte SA, Naranjo CA, Rao HD, Rajurkar MP, Lothe RR, Shaligram US, Johnston RS, Crowell LE, Castelino S, Tracey MK, Whittaker CA, Love JC. Dalvie NC, et al. Among authors: crowell le. bioRxiv [Preprint]. 2021 Apr 15:2021.04.15.440035. doi: 10.1101/2021.04.15.440035. bioRxiv. 2021. PMID: 33880471 Free PMC article. Updated. Preprint.
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters.
Dalvie NC, Rodriguez-Aponte SA, Hartwell BL, Tostanoski LH, Biedermann AM, Crowell LE, Kaur K, Kumru O, Carter L, Yu J, Chang A, McMahan K, Courant T, Lebas C, Lemnios AA, Rodrigues KA, Silva M, Johnston RS, Naranjo CA, Tracey MK, Brady JR, Whittaker CA, Yun D, Kar S, Porto M, Lok M, Andersen H, Lewis MG, Love KR, Camp DL, Silverman JM, Kleanthous H, Joshi SB, Volkin DB, Dubois PM, Collin N, King NP, Barouch DH, Irvine DJ, Love JC. Dalvie NC, et al. Among authors: crowell le. bioRxiv [Preprint]. 2021 Mar 4:2021.03.03.433558. doi: 10.1101/2021.03.03.433558. bioRxiv. 2021. PMID: 33688647 Free PMC article. Updated. Preprint.
14 results