Abstract
Aminoglycoside antibiotics rapidly enter and kill cochlear hair cells via apical mechanoelectrical transduction (MET) channels in vitro. In vivo, it remains unknown whether systemically-administered aminoglycosides cross the blood-labyrinth barrier into endolymph and enter hair cells. Here we show, for the first time, that systemic aminoglycosides are trafficked across the blood-endolymph barrier and preferentially enter hair cells across their apical membranes. This trafficking route is predominant compared to uptake via hair cell basolateral membranes during perilymph infusion.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aminoglycosides / administration & dosage
-
Aminoglycosides / pharmacokinetics*
-
Aminoglycosides / toxicity
-
Animals
-
Biological Transport, Active
-
Endolymph / metabolism*
-
Female
-
Fluorescent Dyes
-
Gentamicins / administration & dosage
-
Gentamicins / pharmacokinetics
-
Gentamicins / toxicity
-
Guinea Pigs
-
Hair Cells, Auditory / drug effects
-
Hair Cells, Auditory / metabolism*
-
Male
-
Models, Biological
-
Perilymph / metabolism
-
Xanthenes
Substances
-
Aminoglycosides
-
Fluorescent Dyes
-
Gentamicins
-
Xanthenes
-
Texas red