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15,249 results

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Page 1
Ligation-based DNA diagnostics.
Landegren U. Landegren U. Bioessays. 1993 Nov;15(11):761-5. doi: 10.1002/bies.950151112. Bioessays. 1993. PMID: 8292008 Review.
A number of novel gene detection techniques all revolve around the ligation of synthetic nucleic acid probes. In such ligase-assisted gene detection reactions, specific DNA or RNA sequences are investigated by using them as guides for the covalent joining of pairs o …
A number of novel gene detection techniques all revolve around the ligation of synthetic nucleic acid probes. In such ligase-assisted …
Click DNA ligation with deoxyribozyme.
Chang Y, Liang Y, Song H, Zhang Q, Yuan H, Li J, Zhang Z, Liu M. Chang Y, et al. Nucleic Acids Res. 2025 Sep 23;53(18):gkaf991. doi: 10.1093/nar/gkaf991. Nucleic Acids Res. 2025. PMID: 41036623 Free PMC article.
Deoxyribozymes that ligate DNA will expand the reaction scope of DNA catalysis and are useful in the construction of DNA nanostructures. ...One remarkable click-ligating deoxyribozyme, named CLDz2, ligated DNA with an observed rate …
Deoxyribozymes that ligate DNA will expand the reaction scope of DNA catalysis and are useful in the construction of …
[DNA assembly by multi-fragment digestion/ligation and homologous recombination].
Rao X, Chong J, He C, Bi Y, Tang G, Lü Y, Gong D, Yang X. Rao X, et al. Sheng Wu Gong Cheng Xue Bao. 2024 May 25;40(5):1559-1570. doi: 10.13345/j.cjb.230793. Sheng Wu Gong Cheng Xue Bao. 2024. PMID: 38783816 Free article. Chinese.
The fragments containing mutations were assembled by introducing mutant sites and Bsa I recognition sequences. After digestion/ligation, the ligated fragment was amplified with the primers containing overlap region to the linearized vector. The amplified fragment wa …
The fragments containing mutations were assembled by introducing mutant sites and Bsa I recognition sequences. After digestion/ligation …
R2D ligase: Unveiling a novel DNA ligase with surprising DNA-to-RNA ligation activity.
Gundesø SE, Rothweiler U, Heimland E, Piotrowski Y, Rødum IK, Söderberg JJ, Gábor IM, Solstad T, Williamson A, Lanes O, Striberny BK. Gundesø SE, et al. Biotechnol J. 2024 Mar;19(3):e2300711. doi: 10.1002/biot.202300711. Biotechnol J. 2024. PMID: 38528369
While DNA ligases preferentially ligate single breaks in double-stranded DNA (dsDNA), they are capable of ligating a multitude of other nucleic acid substrates like blunt-ended dsDNA, TA overhangs, short overhangs and various DNA-RNA hybrids. .. …
While DNA ligases preferentially ligate single breaks in double-stranded DNA (dsDNA), they are capable of ligating …
Highly Effective Chemical Ligation of DNA and l-aTNA.
Okita H, Murayama K, Asanuma H. Okita H, et al. Curr Protoc. 2025 May;5(5):e70140. doi: 10.1002/cpz1.70140. Curr Protoc. 2025. PMID: 40331446
The method uses only N-cyanoimidazole (CNIm) and divalent metal cations to achieve chemical ligation in a template-directed manner, which can be applied to the ligations of not only DNA but also artificial nucleic acids such as acyclic l-threoninol nucleic ac …
The method uses only N-cyanoimidazole (CNIm) and divalent metal cations to achieve chemical ligation in a template-directed manner, w …
Near Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate.
Krishnamurthy K, Rajendran A, Nakata E, Morii T. Krishnamurthy K, et al. Small Methods. 2024 Jan;8(1):e2300999. doi: 10.1002/smtd.202300999. Epub 2023 Sep 21. Small Methods. 2024. PMID: 37736703 Free article.
There have been limited efforts to ligate the staple nicks in DNA origami which is crucial for their stability against thermal and mechanical treatments, and chemical and biological environments. ...Both methods achieved over 90% ligation in 2D origami, only …
There have been limited efforts to ligate the staple nicks in DNA origami which is crucial for their stability against thermal …
Efficient DNA Click Reaction Replaces Enzymatic Ligation.
Kollaschinski M, Sobotta J, Schalk A, Frischmuth T, Graf B, Serdjukow S. Kollaschinski M, et al. Bioconjug Chem. 2020 Mar 18;31(3):507-512. doi: 10.1021/acs.bioconjchem.9b00805. Epub 2020 Jan 8. Bioconjug Chem. 2020. PMID: 31874033
We report a chemical DNA-DNA ligation method based on copper-catalyzed azide-alkyne cycloaddition (CuAAC). ...In comparison to enzymatic ligation methods used to introduce adapters into, e.g., mRNA library preparation, this targeted chemical ligatio …
We report a chemical DNA-DNA ligation method based on copper-catalyzed azide-alkyne cycloaddition (CuAAC). ...In compar …
Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase.
Lohman GJ, Zhang Y, Zhelkovsky AM, Cantor EJ, Evans TC Jr. Lohman GJ, et al. Nucleic Acids Res. 2014 Feb;42(3):1831-44. doi: 10.1093/nar/gkt1032. Epub 2013 Nov 6. Nucleic Acids Res. 2014. PMID: 24203707 Free PMC article.

Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase). PBCV-1 DNA ligase ligated ssDNA splinted by RNA with kcat 8 x 10(-3) s(-1) and K(M) < 1 nM at 25 C under con

…
Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA …
Stabilization and structural changes of 2D DNA origami by enzymatic ligation.
Rajendran A, Krishnamurthy K, Giridasappa A, Nakata E, Morii T. Rajendran A, et al. Nucleic Acids Res. 2021 Aug 20;49(14):7884-7900. doi: 10.1093/nar/gkab611. Nucleic Acids Res. 2021. PMID: 34289063 Free PMC article.
In molecular biology, enzymatic ligation is commonly used to seal the nicks in the duplex DNA. However, in DNA nanotechnology, the ligation procedures are neither optimized for the DNA origami nor routinely applied to link the nicks in it. ...Fu …
In molecular biology, enzymatic ligation is commonly used to seal the nicks in the duplex DNA. However, in DNA nanotech …
Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices.
De Fazio AF, El-Sagheer AH, Kahn JS, Nandhakumar I, Burton MR, Brown T, Muskens OL, Gang O, Kanaras AG. De Fazio AF, et al. ACS Nano. 2019 May 28;13(5):5771-5777. doi: 10.1021/acsnano.9b01294. Epub 2019 Apr 8. ACS Nano. 2019. PMID: 30958671 Free article.
However, the programmability of higher order nanoparticle structures can be easily lost under DNA denaturing conditions. Here, we demonstrate that light can be employed as an external stimulus to master the stability of nanoparticle superlattices (SLs) via the promotion of …
However, the programmability of higher order nanoparticle structures can be easily lost under DNA denaturing conditions. Here, we dem …
15,249 results
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