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DNA Barcoding for the Identification of Botanicals in Herbal Medicine and Dietary Supplements: Strengths and Limitations.
Parveen I, Gafner S, Techen N, Murch SJ, Khan IA. Parveen I, et al. Planta Med. 2016 Sep;82(14):1225-35. doi: 10.1055/s-0042-111208. Epub 2016 Jul 8. Planta Med. 2016. PMID: 27392246 Review.
DNA barcoding, in particular, has been proposed as a means to identify herbal ingredients and to detect adulteration. However, general barcoding techniques using universal primers have been shown to provide mixed results with regard to data accuracy. .
DNA barcoding, in particular, has been proposed as a means to identify herbal ingredients and to detect adulteration. However,
DNA barcoding of populations of Fallopia multiflora, an indigenous herb in China.
Sun XQ, Bai MM, Yao H, Guo JL, Li MM, Hang YY. Sun XQ, et al. Genet Mol Res. 2013 Sep 27;12(3):4078-89. doi: 10.4238/2013.September.27.9. Genet Mol Res. 2013. PMID: 24089097 Free article.
DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plant species. We assessed the applicability of 4 candidate DNA barcodes (matK, rbcL, psbA-trnH, and ITS2) to identify populations
DNA barcoding is a recent aid to taxonomic identification and uses a short standardized DNA region to discriminate plan
Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding.
Cui X, Li W, Wei J, Qi Y, Li R, Yang Y, Shi Y, Meng X, Mi Y, Huot T, Sun W, Zheng X. Cui X, et al. Front Pharmacol. 2020 Mar 24;11:244. doi: 10.3389/fphar.2020.00244. eCollection 2020. Front Pharmacol. 2020. PMID: 32265692 Free PMC article.
In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domestic market. We utilized the DNA barcoding technique, combined with the literature survey, to trace their identities. ...The et …
In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domes …
DNA barcoding of Aristolochia plants and development of species-specific multiplex PCR to aid HPTLC in ascertainment of Aristolochia herbal materials.
Dechbumroong P, Aumnouypol S, Denduangboripant J, Sukrong S. Dechbumroong P, et al. PLoS One. 2018 Aug 20;13(8):e0202625. doi: 10.1371/journal.pone.0202625. eCollection 2018. PLoS One. 2018. PMID: 30125304 Free PMC article.
Any products containing Aristolochia species have thus been banned on many continents, including Europe, America and Asia. Therefore, the development of a method to identify these herbs is critically needed for customer safety. The present study evaluated DNA bar
Any products containing Aristolochia species have thus been banned on many continents, including Europe, America and Asia. Therefore, the de …
DNA Barcoding Identification of Kadsurae Caulis and Spatholobi Caulis Based on Internal Transcribed Spacer 2 Region and Secondary Structure Prediction.
Yu X, Xie Z, Wu J, Tao J, Xu X. Yu X, et al. Pharmacogn Mag. 2016 May;12(Suppl 2):S165-9. doi: 10.4103/0973-1296.182162. Epub 2016 May 11. Pharmacogn Mag. 2016. PMID: 27279702 Free PMC article.
RESULTS: The ITS2 of these two herbs were very different. They were successfully differentiated using the DNA barcoding technique. CONCLUSIONS: DNA barcoding was a promising and reliable tool for the identification of medicinal plants. ...
RESULTS: The ITS2 of these two herbs were very different. They were successfully differentiated using the DNA barcoding
Identification of three Daphne species by DNA barcoding and HPLC fingerprint analysis.
Li Y, Geng L, Liu Y, Chen M, Mu Q, Zhang X, Zhang Z, Ren G, Liu C. Li Y, et al. PLoS One. 2018 Aug 2;13(8):e0201711. doi: 10.1371/journal.pone.0201711. eCollection 2018. PLoS One. 2018. PMID: 30071090 Free PMC article.
In order to well identify the 93 wild Cortex Daphnes samples from different species and habitats in western China and develop a standard operating procedure (SOP) for the authentication and quality of them in the future, a comprehensive and efficient identification system based o …
In order to well identify the 93 wild Cortex Daphnes samples from different species and habitats in western China and develop a standard ope …
DNA barcoding detects contamination and substitution in North American herbal products.
Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S. Newmaster SG, et al. BMC Med. 2013 Oct 11;11:222. doi: 10.1186/1741-7015-11-222. BMC Med. 2013. PMID: 24120035 Free PMC article.
RESULTS: We recovered DNA barcodes from most herbal products (91%) and all leaf samples (100%), with 95% species resolution using a tiered approach (rbcL + ITS2). ...The use of an SRM DNA herbal barcode library for testing bulk materials could provide …
RESULTS: We recovered DNA barcodes from most herbal products (91%) and all leaf samples (100%), with 95% species resolution us …
Mitochondrial plastid DNA can cause DNA barcoding paradox in plants.
Park HS, Jayakodi M, Lee SH, Jeon JH, Lee HO, Park JY, Moon BC, Kim CK, Wing RA, Newmaster SG, Kim JY, Yang TJ. Park HS, et al. Sci Rep. 2020 Apr 9;10(1):6112. doi: 10.1038/s41598-020-63233-y. Sci Rep. 2020. PMID: 32273595 Free PMC article.
The transfer of ancestral plastid genomes into mitochondrial genomes to generate mitochondrial plastid DNA (MTPT) is known to occur in plants, but its impacts on mitochondrial genome complexity and the potential for causing a false-positive DNA barcoding para …
The transfer of ancestral plastid genomes into mitochondrial genomes to generate mitochondrial plastid DNA (MTPT) is known to occur i …
Identification of Chinese Herbs Using a Sequencing-Free Nanostructured Electrochemical DNA Biosensor.
Lei Y, Yang F, Tang L, Chen K, Zhang GJ. Lei Y, et al. Sensors (Basel). 2015 Nov 30;15(12):29882-92. doi: 10.3390/s151229773. Sensors (Basel). 2015. PMID: 26633399 Free PMC article.
By using such a nanofeatured electrochemical DNA (E-DNA) biosensor, two Chinese herbal species of Fritillaria (F. thunbergii and F. cirrhosa) were successfully discriminated at the DNA level, because a fragment of 16-mer sequence at the spacer region of the 5 …
By using such a nanofeatured electrochemical DNA (E-DNA) biosensor, two Chinese herbal species of Fritillaria (F. thunbergii a …
Identification of processed Chinese medicinal materials using DNA mini-barcoding.
Song M, Dong GQ, Zhang YQ, Liu X, Sun W. Song M, et al. Chin J Nat Med. 2017 Jul;15(7):481-486. doi: 10.1016/S1875-5364(17)30073-0. Chin J Nat Med. 2017. PMID: 28807221
Most of Chinese medicinal herbs are subjected to traditional processing procedures, including stir-frying, charring, steaming, boiling, and calcining before they are released into dispensaries. ...In the present study, six DNA barcode loci including IT …
Most of Chinese medicinal herbs are subjected to traditional processing procedures, including stir-frying, charring, steaming, …
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