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Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human.
Wu S, Dalsgaard A, Hammerum AM, Porsbo LJ, Jensen LB. Wu S, et al. Among authors: dalsgaard a. Acta Vet Scand. 2010 Jul 30;52(1):47. doi: 10.1186/1751-0147-52-47. Acta Vet Scand. 2010. PMID: 20670455 Free PMC article.
METHODS: A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. ...Plasmids carrying sul genes were characterized by PCR-based replicon typing to allow a comparison of the types …
METHODS: A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selec …
Cephalosporin-resistant Escherichia coli isolated from farm workers and pigs in northern Vietnam.
Dang STT, Bortolaia V, Tran NT, Le HQ, Dalsgaard A. Dang STT, et al. Among authors: dalsgaard a. Trop Med Int Health. 2018 Apr;23(4):415-424. doi: 10.1111/tmi.13054. Trop Med Int Health. 2018. PMID: 29575455
The ESBL phenotype was mainly mediated by CTX-M and to a lesser extent by TEM. Occurrence of bla(CTX)(-M) was similar in E. coli from pigs (66.7%) and humans (68.5%). CONCLUSION: The high occurrence of ESBL-producing E. coli in pig farmers and pigs could present a r …
The ESBL phenotype was mainly mediated by CTX-M and to a lesser extent by TEM. Occurrence of bla(CTX)(-M) was similar in E. coli from …
Diarrheagenic Escherichia coli and Shigella strains isolated from children in a hospital case-control study in Hanoi, Vietnam.
Hien BT, Scheutz F, Cam PD, Serichantalergs O, Huong TT, Thu TM, Dalsgaard A. Hien BT, et al. Among authors: dalsgaard a. J Clin Microbiol. 2008 Mar;46(3):996-1004. doi: 10.1128/JCM.01219-07. Epub 2008 Jan 3. J Clin Microbiol. 2008. PMID: 18174300 Free PMC article.
Serotyping was used to distinguish between A/EEC and EPEC. However, A/EEC, EPEC, and EAggEC were isolated at high frequency from both cases and controls. Further in-depth studies are needed to better understand important virulence factors of DEC, especially A
Serotyping was used to distinguish between A/EEC and EPEC. However, A/EEC, EPEC, and EAggEC were isolated at high frequency fr …
Fate of CMY-2-Encoding Plasmids Introduced into the Human Fecal Microbiota by Exogenous Escherichia coli.
Anjum M, Madsen JS, Nesme J, Jana B, Wiese M, Jasinskytė D, Nielsen DS, Sørensen SJ, Dalsgaard A, Moodley A, Bortolaia V, Guardabassi L. Anjum M, et al. Among authors: dalsgaard a. Antimicrob Agents Chemother. 2019 Apr 25;63(5):e02528-18. doi: 10.1128/AAC.02528-18. Print 2019 May. Antimicrob Agents Chemother. 2019. PMID: 30885897 Free PMC article.
The gut is a hot spot for transfer of antibiotic resistance genes from ingested exogenous bacteria to the indigenous microbiota. The objective of this study was to determine the fate of two nearly identical bla (CMY-2)-harboring plasmids introduced into the human fecal mic …
The gut is a hot spot for transfer of antibiotic resistance genes from ingested exogenous bacteria to the indigenous microbiota. The …
Development and evaluation of novel one-step TaqMan realtime RT-PCR assays for the detection and direct genotyping of genogroup I and II noroviruses.
Schultz AC, Vega E, Dalsgaard A, Christensen LS, Nørrung B, Hoorfar J, Vinjé J. Schultz AC, et al. Among authors: dalsgaard a. J Clin Virol. 2011 Mar;50(3):230-4. doi: 10.1016/j.jcv.2010.12.001. Epub 2010 Dec 30. J Clin Virol. 2011. PMID: 21195660
BACKGROUND: Current detection and genotyping methods of genogroup (G) I and II noroviruses (NoVs) consist of a 2-step approach including detection of viral RNA by TaqMan realtime RT-PCR (RT-qPCR) followed by conventional RT-PCR and sequencing of partial regions of ORF1 or …
BACKGROUND: Current detection and genotyping methods of genogroup (G) I and II noroviruses (NoVs) consist of a 2-step approach includ …
Evaluation of Petrifilm Select E. coli Count Plate medium to discriminate antimicrobial resistant Escherichia coli.
Wu S, Chouliara E, Jensen LB, Dalsgaard A. Wu S, et al. Among authors: dalsgaard a. Acta Vet Scand. 2008 Sep 25;50(1):38. doi: 10.1186/1751-0147-50-38. Acta Vet Scand. 2008. PMID: 18817575 Free PMC article.
High agreement levels were found for gentamicin, ampicillin, chloramphenicol and cefotaxime, resulting in a kappa value of 0.9 and 100% agreement within +/- 1 log2 dilution. ...SEC plates can also be used to discriminate oxytetracycline-resistant E. coli if a plate- …
High agreement levels were found for gentamicin, ampicillin, chloramphenicol and cefotaxime, resulting in a kappa value of 0.9 and 10 …
Prevalence of tetracycline resistance and genotypic analysis of populations of Escherichia coli from animals, carcasses and cuts processed at a pig slaughterhouse.
Wu S, Dalsgaard A, Vieira AR, Emborg HD, Jensen LB. Wu S, et al. Among authors: dalsgaard a. Int J Food Microbiol. 2009 Nov 15;135(3):254-9. doi: 10.1016/j.ijfoodmicro.2009.07.027. Epub 2009 Aug 4. Int J Food Microbiol. 2009. PMID: 19717199
A Danish pig slaughterhouse was visited in this study to investigate the impact of carcass processing on prevalence of tetracycline-resistant Escherichia coli, and to identify the origins of carcass contaminations with E. coli by assessing genetic diversity of E. coli popu
A Danish pig slaughterhouse was visited in this study to investigate the impact of carcass processing on prevalence of tetracycline-r
Transmission of antibiotic-resistant Escherichia coli between cattle, humans and the environment in peri-urban livestock keeping communities in Morogoro, Tanzania.
Lupindu AM, Dalsgaard A, Msoffe PL, Ngowi HA, Mtambo MM, Olsen JE. Lupindu AM, et al. Among authors: dalsgaard a. Prev Vet Med. 2015 Mar 1;118(4):477-82. doi: 10.1016/j.prevetmed.2014.12.005. Epub 2014 Dec 15. Prev Vet Med. 2015. PMID: 25555902
Such farming practices pose a public health risk as livestock are reservoirs of several zoonotic pathogens. In an attempt to determine the fecal transmission between livestock and people, 100 household clusters keeping cattle in close proximity of humans were selected in u …
Such farming practices pose a public health risk as livestock are reservoirs of several zoonotic pathogens. In an attempt to determin …
Cryptosporidium and Giardia in Danish organic pig farms: Seasonal and age-related variation in prevalence, infection intensity and species/genotypes.
Petersen HH, Jianmin W, Katakam KK, Mejer H, Thamsborg SM, Dalsgaard A, Olsen A, Enemark HL. Petersen HH, et al. Among authors: dalsgaard a. Vet Parasitol. 2015 Nov 30;214(1-2):29-39. doi: 10.1016/j.vetpar.2015.09.020. Epub 2015 Sep 21. Vet Parasitol. 2015. PMID: 26483166
Of the successfully genotyped isolates, 38/56 (67.9%) were C. scrofarum and 18/56 (32.1%) were C. suis, while the livestock specific G. duodenalis Assemblage E was detected in 11/13 (84.6%) isolates and the potentially zoonotic Assemblage A was identified in 2/13 (15.4%) i …
Of the successfully genotyped isolates, 38/56 (67.9%) were C. scrofarum and 18/56 (32.1%) were C. suis, while the livestock specific G. duod …
Identification and Antimicrobial Resistance of Bacteria Isolated from Probiotic Products Used in Shrimp Culture.
Noor Uddin GM, Larsen MH, Christensen H, Aarestrup FM, Phu TM, Dalsgaard A. Noor Uddin GM, et al. Among authors: dalsgaard a. PLoS One. 2015 Jul 6;10(7):e0132338. doi: 10.1371/journal.pone.0132338. eCollection 2015. PLoS One. 2015. PMID: 26147573 Free PMC article.
MIC testing was done for a range of antimicrobials and whole genome sequencing of six multiple antimicrobial resistant Bacillus spp. used to identify resistance genes and genetic elements associated with horizontal gene transfer. ...Although our culture-based isolation and …
MIC testing was done for a range of antimicrobials and whole genome sequencing of six multiple antimicrobial resistant Bacillus spp. …
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