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Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.
Darzins A. Darzins A. Mol Microbiol. 1994 Jan;11(1):137-53. doi: 10.1111/j.1365-2958.1994.tb00296.x. Mol Microbiol. 1994. PMID: 7908398
The type 4 pili of Pseudomonas aeruginosa are important cell-associated virulence factors that play a crucial role in mediating (i) bacterial adherence to, and colonization of, mucosal surfaces, (ii) a novel mode of flagella-independent surface translocation known a …
The type 4 pili of Pseudomonas aeruginosa are important cell-associated virulence factors that play a crucial role in mediating (i) b …
Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.
Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS. Whitchurch CB, et al. Among authors: darzins a. Mol Microbiol. 2004 May;52(3):873-93. doi: 10.1111/j.1365-2958.2004.04026.x. Mol Microbiol. 2004. PMID: 15101991 Free article.
Clustering of mutations affecting alginic acid biosynthesis in mucoid Pseudomonas aeruginosa.
Darzins A, Wang SK, Vanags RI, Chakrabarty AM. Darzins A, et al. J Bacteriol. 1985 Nov;164(2):516-24. doi: 10.1128/JB.164.2.516-524.1985. J Bacteriol. 1985. PMID: 3932325 Free PMC article.
A 10-kilobase DNA fragment previously shown to contain the phosphomannose isomerase gene (pmi) of Pseudomonas aeruginosa was used to construct a pBR325-based hybrid that can be propagated in P. aeruginosa only by the formation of a chromosomal-plasmid cointeg
A 10-kilobase DNA fragment previously shown to contain the phosphomannose isomerase gene (pmi) of Pseudomonas aeruginosa was used to
Gluconeogenic mutations in Pseudomonas aeruginosa: genetic linkage between fructose-bisphosphate aldolase and phosphoglycerate kinase.
Banerjee PC, Darzins A, Maitra PK. Banerjee PC, et al. J Gen Microbiol. 1987 Apr;133(4):1099-107. doi: 10.1099/00221287-133-4-1099. J Gen Microbiol. 1987. PMID: 3116166
Partial restoration of FBA activity in spontaneous revertants of Fba- mutants was accompanied by a concomitant loss of PGK activity. These experiments indicate a linkage between the fba and pgk genes on the P. aeruginosa chromosome....
Partial restoration of FBA activity in spontaneous revertants of Fba- mutants was accompanied by a concomitant loss of PGK activity. …
Characterization of the Pseudomonas aeruginosa alginate (alg) gene region II.
Wang SK, Sa'-Correia I, Darzins A, Chakrabarty AM. Wang SK, et al. J Gen Microbiol. 1987 Aug;133(8):2303-14. doi: 10.1099/00221287-133-8-2303. J Gen Microbiol. 1987. PMID: 3127542
Overexpression of the genes controlling alginate biosynthesis within region II was accomplished by placing various cloned restriction fragments under the transcriptional control of the hybrid trp-lac (tac) promoter, and plasmid encoded proteins were examined in a maxicell …
Overexpression of the genes controlling alginate biosynthesis within region II was accomplished by placing various cloned restriction fragme …
Molecular genetic analysis of type-4 pilus biogenesis and twitching motility using Pseudomonas aeruginosa as a model system--a review.
Darzins A, Russell MA. Darzins A, et al. Gene. 1997 Jun 11;192(1):109-15. doi: 10.1016/s0378-1119(97)00037-1. Gene. 1997. PMID: 9224880 Review.
One pil locus, designated pilE, resides at approx. 71 min on the PAO genetic map, a region of the chromosome previously shown to harbor a number of genes required for pilus assembly (i.e., pilA, -B, -C, -D, -R and -S). ...Included within this homologous region is …
One pil locus, designated pilE, resides at approx. 71 min on the PAO genetic map, a region of the chromosome previously shown to harb …
Pseudomonas aeruginosa lasA gene: determination of the transcription start point and analysis of the promoter/regulatory region.
Freck-O'Donnell LC, Darzins A. Freck-O'Donnell LC, et al. Gene. 1993 Jul 15;129(1):113-7. doi: 10.1016/0378-1119(93)90705-8. Gene. 1993. PMID: 8335250
The DNA sequence of the region upstream from the tsp was determined, and a putative sigma promoter was identified. Sequence comparison with the lasB promoter region revealed two areas of considerable homology which could act as potential binding sites for LasR or other, as …
The DNA sequence of the region upstream from the tsp was determined, and a putative sigma promoter was identified. Sequence compariso …
The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.
Darzins A. Darzins A. J Bacteriol. 1993 Sep;175(18):5934-44. doi: 10.1128/jb.175.18.5934-5944.1993. J Bacteriol. 1993. PMID: 8104179 Free PMC article.
The Pseudomonas aeruginosa pilG gene, encoding a protein which is involved in pilus production, was cloned by phenotypic complementation of a unique, pilus-defective mutant of strain PAO1. ...A 700-bp region encompassing the pilG gene was sequenced, and a
The Pseudomonas aeruginosa pilG gene, encoding a protein which is involved in pilus production, was cloned by phenotypic complementat …
The pilE gene product of Pseudomonas aeruginosa, required for pilus biogenesis, shares amino acid sequence identity with the N-termini of type 4 prepilin proteins.
Russell MA, Darzins A. Russell MA, et al. Mol Microbiol. 1994 Sep;13(6):973-85. doi: 10.1111/j.1365-2958.1994.tb00489.x. Mol Microbiol. 1994. PMID: 7854130
Cloning of the pilE gene was facilitated by the isolation and identification of a pro(B+)-containing plasmid from a PAO1 cosmid library. ...PilE, a protein with a molecular weight of 15,278, showed significant sequence identity to the pilin precursors …
Cloning of the pilE gene was facilitated by the isolation and identification of a pro(B+)-containing plasmid from a PAO1 cosmi …
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