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Peptidyl-tRNA hydrolase and its critical role in protein biosynthesis.
Das G, Varshney U. Das G, et al. Microbiology. 2006 Aug;152(Pt 8):2191-5. doi: 10.1099/mic.0.29024-0. Microbiology. 2006. PMID: 16849786 Review.
Evidence for a role of initiation factor 3 in recycling of ribosomal complexes stalled on mRNAs in Escherichia coli.
Singh NS, Das G, Seshadri A, Sangeetha R, Varshney U. Singh NS, et al. Nucleic Acids Res. 2005 Sep 30;33(17):5591-601. doi: 10.1093/nar/gki864. Print 2005. Nucleic Acids Res. 2005. PMID: 16199751 Free PMC article.
Specific interactions between ribosome recycling factor (RRF) and elongation factor-G (EFG) mediate disassembly of post-termination ribosomal complexes for new rounds of initiation. ...
Specific interactions between ribosome recycling factor (RRF) and elongation factor-G (EFG) mediate disassembly of post-termination r …
An extended Shine-Dalgarno sequence in mRNA functionally bypasses a vital defect in initiator tRNA.
Shetty S, Nadimpalli H, Shah RA, Arora S, Das G, Varshney U. Shetty S, et al. Proc Natl Acad Sci U S A. 2014 Oct 7;111(40):E4224-33. doi: 10.1073/pnas.1411637111. Epub 2014 Sep 22. Proc Natl Acad Sci U S A. 2014. PMID: 25246575 Free PMC article.
Initiator tRNAs are special in their direct binding to the ribosomal P-site due to the hallmark occurrence of the three consecutive G-C base pairs (3GC pairs) in their anticodon stems. ...
Initiator tRNAs are special in their direct binding to the ribosomal P-site due to the hallmark occurrence of the three consecutive G
Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli.
Kapoor S, Das G, Varshney U. Kapoor S, et al. Nucleic Acids Res. 2011 Jan;39(1):202-12. doi: 10.1093/nar/gkq760. Epub 2010 Aug 26. Nucleic Acids Res. 2011. PMID: 20798174 Free PMC article.
A highly conserved occurrence of three consecutive G-C base pairs in the anticodon stem of tRNA(fMet) contributes to its preferential selection in the P-site. In a genetic screen, using a plasmid borne copy of an inactive tRNA(fMet) mutant wherein the three G-C base …
A highly conserved occurrence of three consecutive G-C base pairs in the anticodon stem of tRNA(fMet) contributes to its preferential …
Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli.
Das G, Thotala DK, Kapoor S, Karunanithi S, Thakur SS, Singh NS, Varshney U. Das G, et al. EMBO J. 2008 Mar 19;27(6):840-51. doi: 10.1038/emboj.2008.20. Epub 2008 Feb 21. EMBO J. 2008. PMID: 18288206 Free PMC article.
Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. ...Using a geneti …
Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature …
A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors.
Gaur R, Grasso D, Datta PP, Krishna PD, Das G, Spencer A, Agrawal RK, Spremulli L, Varshney U. Gaur R, et al. Mol Cell. 2008 Feb 1;29(2):180-90. doi: 10.1016/j.molcel.2007.11.021. Mol Cell. 2008. PMID: 18243113 Free PMC article.
Expression of human gelatinase B in Pichia pastoris.
Roy N, Padmanabhan S, Smith M, Shi L, Navre M, Das G. Roy N, et al. Protein Expr Purif. 1999 Jul;16(2):324-30. doi: 10.1006/prep.1999.1073. Protein Expr Purif. 1999. PMID: 10419828
Production of recombinant human bile salt stimulated lipase and its variant in Pichia pastoris.
Sahasrabudhe AV, Solapure SM, Khurana R, Suryanarayan V, Ravishankar S, deSousa SM, Das G. Sahasrabudhe AV, et al. Protein Expr Purif. 1998 Dec;14(3):425-33. doi: 10.1006/prep.1998.0974. Protein Expr Purif. 1998. PMID: 9882578
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